ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2020
Developmentally Engineered Callus Organoid Bioassemblies Exhibit Predictive In Vivo Long Bone Healing.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
99 citing papers in PubMed, 207 citations in OpenAlex.
- Bone organoids and mitochondrial reprogramming.Journal of orthopaedic translation · 2026Review
- Emerging engineering strategies in bone organoids: From biomimetic scaffolds to dynamic microenvironmental stimulation.Bioactive materials · 2026Review
- Packed for Ossification: High-Density Bioprinting of hPDC Spheroids in HAMA Toward Endochondral Ossification.Advanced healthcare materials · 2026Article
- From microtissues to macro solutions - The future of scalable and automated cartilage tissue engineering.Journal of orthopaedic translation · 2026Review
- Gli1⁺ cell aggregates promote type H vessel formation and orchestrate bone defect regeneration.Stem cell research & therapy · 2026Article
- Synchronizing the Osteochondral Regeneration Process through Spatial Patterning of Stable and Hypertrophic Cartilage Organoids.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Recapitulating Endochondral Ossification for Bone Repair: From Development to Engineering Strategy.Advanced healthcare materials · 2026Review
- Understanding the use of spheroids and pellets in the chondrogenic differentiation of human stem cells.Osteoarthritis and cartilage open · 2026Article
- Periosteal mitochondria DNA structures drive aging-associated poor skeletal repair.Bone research · 2026Article
- Bone Organoids: A Novel Tool for Modeling and Managing Skeletal Disorders in Diabetes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Start, Stop, Rewind, Repeat-Cyclic Exposure of Adipose Stromal Cells-derived Cartilage Organoids to Chondrogenic and Proliferative Cues to Achieve Scaled-up and Customizable Bone Formation by Endochondral Ossification.Advanced healthcare materials · 2026Article
- Stromal vascular fraction self-assembles vascularized osteogenic organoids with immunomodulatory functions.Bioactive materials · 2026Article
- Advancements in bone organoids: perspectives on construction methodologies and application strategies.Journal of advanced research · 2026Review
- Dynamic microenvironments for bone organoids: spatiotemporal regulation of angiogenic-osteogenic coupling through advanced hydrogel design.Frontiers in bioengineering and biotechnology · 2026Review
- Engineered 3D mesenchymal stem cell aggregates with multifunctional prowess for bone regeneration: Current status and future prospects.Journal of advanced research · 2026Review
- Organoids in Cancer Research and Regenerative Medicine: Current Status, Challenges, and Future Prospects.MedComm · 2026Review
- Review
- Active foam dynamics of tissue spheroid fusion.Nature communications · 2025Article
- Synergistic promotion of bone regeneration through co-culture of endothelial cells with mesenchymal stem cells in endochondral ossification organoids.Stem cell research & therapy · 2025Article
- 3D bioprinting bone/cartilage organoids: construction, applications, and challenges.Journal of orthopaedic translation · 2025Review
39 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clinical translation of cell-based products is hampered by their limited predictive in vivo performance. To overcome this hurdle, engineering strategies advocate to fabricate tissue products through processes that mimic development and regeneration, a strategy applicable for the healing of large bone defects, an unmet medical need. Natural fracture healing occurs through the formation of a cartilage intermediate, termed "soft callus," which is transformed into bone following a process that recapitulates developmental events. The main contributors to the soft callus are cells derived from the periosteum, containing potent skeletal stem cells. Herein, cells derived from human periosteum are used for the scalable production of microspheroids that are differentiated into callus organoids. The organoids attain autonomy and exhibit the capacity to form ectopic bone microorgans in vivo. This potency is linked to specific gene signatures mimicking those found in developing and healing long bones. Furthermore, callus organoids spontaneously bioassemble in vitro into large engineered tissues able to heal murine critical-sized long bone defects. The regenerated bone exhibits similar morphological properties to those of native tibia. These callus organoids can be viewed as a living "bio-ink" allowing bottom-up manufacturing of multimodular tissues with complex geometric features and inbuilt quality attributes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.