ReviewApplications in plant sciences2020
Considerations in adapting CRISPR/Cas9 in nongenetic model plant systems.
Review in Applications in plant sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Genome Editing Approaches in Flax (International journal of molecular sciences · 2026Review
- First Successful Targeted Mutagenesis Using CRISPR/Cas9 in Stably Transformed Grain Amaranth Tissue.Plant biotechnology journal · 2026Article
- Walnut genome editing: an optimized CRISPR/Cas9 platform with superior genotypes and endogenous promoters.Horticulture research · 2025Article
- Comprehensive benchmarking of genome editing quantification methods for plant applications.iScience · 2025Article
- DNA-free CRISPR genome editing in raspberry (Frontiers in genome editing · 2025Article
- Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency.Biotechnology journal · 2024Review
- Plant conservation in the age of genome editing: opportunities and challenges.Genome biology · 2024Review
- Review
- Can genome editing help transitioning to agroecology?iScience · 2024Review
- The Role of Plant Latex in Virus Biology.Viruses · 2023Review
- Strategies and Methods for Improving the Efficiency of CRISPR/Cas9 Gene Editing in Plant Molecular Breeding.Plants (Basel, Switzerland) · 2023Review
- Facilitated Adaptation as A Conservation Tool in the Present Climate Change Context: A Methodological Guide.Plants (Basel, Switzerland) · 2023Review
- Branching Ionizable Lipids Can Enhance the Stability, Fusogenicity, and Functional Delivery of mRNA.Small science · 2023Article
- Knockout of elF4E using CRISPR/Cas9 for large-scale production of resistant cucumber cultivar against WMV, ZYMV, and PRSV.Frontiers in plant science · 2023Article
- Fine-tuning CRISPR/Cas9 gene editing in common bean (Frontiers in plant science · 2023Article
- Exploring the Agrobacterium-mediated transformation with CRISPR/Cas9 in cucumber (Cucumis sativus L.).Molecular biology reports · 2022Article
- Virus-induced gene silencing for in planta validation of gene function in cucurbits.Plant physiology · 2022Article
- Function Analysis of theInternational journal of molecular sciences · 2022Article
- Optimization of Genome Knock-In Method: Search for the Most Efficient Genome Regions for Transgene Expression in Plants.International journal of molecular sciences · 2022Review
- The biology ofeLife · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The past six years have seen the rapid growth of studies of CRISPR/Cas9 in plant genome editing, a method that enormously facilitates both basic research and practical applications. Most studies have focused on genetic model species, but plant species that are not genetic models may also be economically important or biologically significant, or both. However, developing the CRISPR/Cas9 system in a nongenetic model is challenging. Here, we summarize CRISPR/Cas9 applications in 45 plant genera across 24 families and provide a reference for practical application of CRISPR in nongenetic model plant systems. Suggestions for selecting plant species and target genes are given for proof-of-principle CRISPR studies, and the processes of vector construction are reviewed. We recommend using transient assays to identify a desired CRISPR/Cas9 system in a nongenetic model. We then review methods of plant transformation and describe approaches, using regenerated transgenic plants, for evaluating CRISPR editing results. Lastly, potential future applications of CRISPR in nongenetic model plant species are discussed. This review provides a road map for developing CRISPR in nongenetic models, an application that holds enormous potential in plant biology.
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