ArticleBMC infectious diseases2020
Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection.
Article in BMC infectious diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 53 citations in OpenAlex.
- Establishment and application of a rapid and visual detection method forBiosafety and health · 2026Article
- Exploiting the Clinical Potential of a Chemiluminescent Probe forChemical & biomedical imaging · 2026Article
- Rapid Detection ofMicroorganisms · 2026Article
- A one-pot, visual, multiplex microfluidic digital PCR chip for simultaneous point-of-care detection, genotyping, and macrolide resistance assessment of Mycoplasma pneumoniae.Mikrochimica acta · 2025Article
- The establishment and optimization of aMicrobiology spectrum · 2025Article
- Establishment and Application of Duplex Recombinase-Aided Amplification Combined with Lateral Flow Dipsticks for Rapid and Simultaneous Visual Detection ofFoods (Basel, Switzerland) · 2025Article
- Rapid and facile detection of Mycoplasma pneumoniae 16S rRNA based on CHA-FICA system.Mikrochimica acta · 2025Article
- Rapid detection ofFrontiers in cellular and infection microbiology · 2025Article
- Laboratory diagnosis and treatment ofAnnals of medicine · 2024Review
- Detection method for reverse transcription recombinase-aided amplification of avian influenza virus subtypes H5, H7, and H9.BMC veterinary research · 2024Article
- Laboratory tools for the direct detection of bacterial respiratory infections and antimicrobial resistance: a scoping review.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Recombinase-based amplification coupled with lateral flow chromatography for the specific and sensitive detection and identification ofFrontiers in microbiology · 2024Article
- An accurate and convenient method forFrontiers in cellular and infection microbiology · 2024Article
- Review
- Real-time recombinase-aided amplification assay for rapid amplification of the IS1081 gene of Mycobacterium tuberculosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023Article
- Development of a Novel Method for the Clinical Visualization and Rapid Identification of Multidrug-Resistant Candida auris.Microbiology spectrum · 2023Article
- Imunocapture Magnetic Beads Enhanced and Ultrasensitive CRISPR-Cas13a-Assisted Electrochemical Biosensor for Rapid Detection of SARS-CoV-2.Biosensors · 2023Article
- Establishment and application of the Recombinase-Aided Amplification-Lateral Flow Dipstick detection method forAustralasian plant pathology : APP · 2023Article
- Advances in the application of recombinase-aided amplification combined with CRISPR-Cas technology in quick detection of pathogenic microbes.Frontiers in bioengineering and biotechnology · 2023Review
- Absolute quantification ofFrontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundMycoplasma pneumoniae is one of the most common causative pathogens of community-acquired pneumonia (CAP), accounting for as many as 30-50% of CAP during peak years. An early and rapid diagnostic method is key for guiding clinicians in their choice of antibiotics.
methodsThe recombinase-aided amplification (RAA) assay is a recently developed, rapid detection method that has been used for the detection of several pathogens. The assays were performed in a one-step single tube reaction at 39° Celsius within 15-30 min. In this study, we established an RAA assay for M. pneumoniae using clinical specimens for validation and commercial real-time PCR as the reference method.
resultsThe analytical sensitivity of the RAA assay was 2.23 copies per reaction, and no cross-reactions with any of the other 15 related respiratory bacterial pathogens were observed. Compared with the commercial real-time PCR assay used when testing 311 respiratory specimens, the RAA assay obtained 100% sensitivity and 100% specificity with a kappa value of 1.
conclusionsThese results demonstrate that the proposed RAA assay will be of benefit as a faster, sensitive, and specific alternative tool for the detection of M. pneumoniae.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.