Evidence map›Paper›PMID 31991740›Full record

ArticleCancers2020

GPER Activation Inhibits Cancer Cell Mechanotransduction and Basement Membrane Invasion via RhoA.

Alistair Rice, Ernesto Cortes, Dariusz Lachowski, Philipp Oertle, Carlos Matellan, Stephen D Thorpe, Ritobrata Ghose, Haiyun Wang, David A Lee, Marija Plodinec and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancers, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
1.8field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Article
  6. Female Anterior Cruciate Ligaments Exhibit a Muted Mechanobiological Response to Mechanical Loading.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025
    Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Review
  12. Review
  13. Article
  14. Review
  15. Review
  16. Article
  17. Effects ofMolecules (Basel, Switzerland) · 2021
    Article
  18. Review
  19. GPER and Testicular Germ Cell Cancer.Frontiers in endocrinology · 2020
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 4 countries.

Alistair RiceCellular and Molecular Biomechanics Laboratory, Department of Bioengineering, Faculty of Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Ernesto CortesCellular and Molecular Biomechanics Laboratory, Department of Bioengineering, Faculty of Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Dariusz LachowskiCellular and Molecular Biomechanics Laboratory, Department of Bioengineering, Faculty of Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Philipp OertleBiozentrum and the Swiss Nanoscience Institute, University of Basel, 4056 Basel, Switzerland.
Carlos MatellanCellular and Molecular Biomechanics Laboratory, Department of Bioengineering, Faculty of Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Stephen D ThorpeInstitute of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, UK.ORCID 0000-0002-4707-7756
Ritobrata GhoseCellular and Molecular Biomechanics Laboratory, Department of Bioengineering, Faculty of Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Haiyun WangSchool of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
David A LeeInstitute of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, UK.
Marija PlodinecBiozentrum and the Swiss Nanoscience Institute, University of Basel, 4056 Basel, Switzerland.
Armando E Del Río HernándezCellular and Molecular Biomechanics Laboratory, Department of Bioengineering, Faculty of Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Imperial College London · GBQueen Mary University of London · GBUniversity of Basel · CHCentre for Genomic Regulation · ESTongji University · CN

Funding

Biotechnology and Biological Sciences Research Council BB/N018532/1European Research Council 282051
6 · The paper itself

Abstract

The invasive properties of cancer cells are intimately linked to their mechanical phenotype, which can be regulated by intracellular biochemical signalling. Cell contractility, induced by mechanotransduction of a stiff fibrotic matrix, and the epithelial-mesenchymal transition (EMT) promote invasion. Metastasis involves cells pushing through the basement membrane into the stroma-both of which are altered in composition with cancer progression. Agonists of the G protein-coupled oestrogen receptor (GPER), such as tamoxifen, have been largely used in the clinic, and interest in GPER, which is abundantly expressed in tissues, has greatly increased despite a lack of understanding regarding the mechanisms which promote its multiple effects. Here, we show that specific activation of GPER inhibits EMT, mechanotransduction and cell contractility in cancer cells via the GTPase Ras homolog family member A (RhoA). We further show that GPER activation inhibits invasion through an in vitro basement membrane mimic, similar in structure to the pancreatic basement membrane that we reveal as an asymmetric bilayer, which differs in composition between healthy and cancer patients.

Indexed as

cancer biomechanicsG protein-coupled receptorsmetastasistumour microenvironment

Identifiers

PMID31991740
PMCPMC7073197
OpenAlexW3002692285

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.