ArticleeLife2020
Multiplex CRISPR/Cas screen in regenerating haploid limbs of chimeric Axolotls.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Signal control during tissue regeneration in adult animals.Nature reviews. Molecular cell biology · 2026Review
- Article
- Optimized toolkit for the manipulation of immortalized axolotl fibroblasts.Methods (San Diego, Calif.) · 2025Article
- Annelids as models of germ cell and gonad regeneration.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Review
- A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Rebuilding limbs, one cell at a time.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- The amazing and anomalous axolotls as scientific models.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- MAPK/ERK Pathway as a Central Regulator in Vertebrate Organ Regeneration.International journal of molecular sciences · 2022Review
- The Axolotl's journey to the modern molecular era.Current topics in developmental biology · 2022Article
- Article
- Salamanders: The molecular basis of tissue regeneration and its relevance to human disease.Current topics in developmental biology · 2021Review
- What the salamander eye has been telling the vision scientist's brain.Seminars in cell & developmental biology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Axolotls and other salamanders can regenerate entire limbs after amputation as adults, and much recent effort has sought to identify the molecular programs controlling this process. While targeted mutagenesis approaches like CRISPR/Cas9 now permit gene-level investigation of these mechanisms, genetic screening in the axolotl requires an extensive commitment of time and space. Previously, we quantified CRISPR/Cas9-generated mutations in the limbs of mosaic mutant axolotls before and after regeneration and found that the regenerated limb is a highfidelity replicate of the original limb (Flowers et al. 2017). Here, we circumvent aforementioned genetic screening limitations and present methods for a multiplex CRISPR/Cas9 haploid screen in chimeric axolotls (MuCHaChA), which is a novel platform for haploid genetic screening in animals to identify genes essential for limb regeneration.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.