Evidence map›Paper›PMID 31989556›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Using a Lentivirus-Based Inducible RNAi Vector to Silence a Gene.

Si Chen, Dongying Li, Zhen Ren, Dianke Yu, Baitang Ning, Nan Mei, Lei Guo

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Si ChenDivision of Biochemical Toxicology, National Center for Toxicological Research (NCTR)/U.S. FDA, Jefferson, AR, USA. si.chen@fda.hhs.gov.
Dongying LiDivision of Bioinformatics and Biostatistics, National Center for Toxicological Research (NCTR)/U.S. FDA, Jefferson, AR, USA.
Zhen RenDivision of Biochemical Toxicology, National Center for Toxicological Research (NCTR)/U.S. FDA, Jefferson, AR, USA.
Dianke YuDivision of Bioinformatics and Biostatistics, National Center for Toxicological Research (NCTR)/U.S. FDA, Jefferson, AR, USA.
Baitang NingDivision of Bioinformatics and Biostatistics, National Center for Toxicological Research (NCTR)/U.S. FDA, Jefferson, AR, USA.
Nan MeiDivision of Molecular and Genetic Toxicology, National Center for Toxicological Research (NCTR)/U.S. FDA, Jefferson, AR, USA.
Lei GuoDivision of Biochemical Toxicology, National Center for Toxicological Research (NCTR)/U.S. FDA, Jefferson, AR, USA.
National Center for Toxicological Research · USUnited States Food and Drug Administration · US

Funding

Intramural FDA HHS FD999999
6 · The paper itself

Abstract

RNA interference (RNAi) is a powerful approach for inhibiting gene expression and its wide applications have expanded our understanding of gene functions. Short hairpin RNAs (shRNAs) are artificially synthesized RNA molecules used to mediate RNAi. The expression of shRNA in cells can be achieved by using plasmids or viral/bacterial vectors. The use of viral vectors to carry and deliver shRNA shows many advantages, including the ability to overcome the difficulty of transfecting certain cell types, the capability to establish stable cell lines via antibiotics selection or fluorescence-activated cell sorting, and the option to control temporally shRNA expression using inducible promoters. In this chapter, we introduce a gene silencing method utilizing a lentivirus-based inducible shRNA system. Using the human topoisomerase (TOP) gene as an example, we describe a procedure to generate stable HepG2 cells showing inducible suppression of TOP1. In addition, a procedure for assessing the efficiency of gene silencing is described in detail.

Indexed as

RNA InterferenceDNA Topoisomerases, Type IGene Knockdown TechniquesGenetic VectorsHep G2 CellsHumansLentivirusRNA, Small InterferingWorkflowDNA Topoisomerases, Type IRNA, Small InterferingGene silencingLentivirusRNAishRNATopoisomerase1

Identifiers

PMID31989556
PMCPMC12418719
OpenAlexW3003549804

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.