ArticleMolecular biology reports2020
Protocol for purification and culture of astrocytes: useful not only in 2 days postnatal but also in adult rat brain.
Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 19 citations in OpenAlex.
- TTP protects against 6-OHDA-induced dopaminergic neurodegeneration via astrocytic Nrf2/HO-1 activation in cellular and rat models of Parkinson's disease.Metabolic brain disease · 2026Article
- Ptbp1 knockdown induces conversion of rat spinal cord astrocytes into neuron like cells.Scientific reports · 2025Article
- Article
- miR-193b-3p and miR-346 Exert Antihypertensive Effects in the Rostral Ventrolateral Medulla.Journal of the American Heart Association · 2024Article
- Targeting reactive astrocytes by pH-responsive ligand-bonded polymeric nanoparticles in spinal cord injury.Drug delivery and translational research · 2023Article
- Understanding astrocyte differentiation: Clinical relevance, technical challenges, and new opportunities in the omics era.WIREs mechanisms of disease · 2022Review
- Thymoquinone Improved Nonylphenol-Induced Memory Deficit and Neurotoxicity Through Its Antioxidant and Neuroprotective Effects.Molecular neurobiology · 2022Article
- In vitro characterization of subventricular zone isolated neural stem cells, from adult monkey and rat brain.Molecular biology reports · 2021Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Astrocytes play the key roles in the physiology and pathology of the CNS. Thereupon, in this manuscript, we aim to demonstrate that the protocol for purification and culture of astrocytes is useful not only in 2 days postnatal but also in adult rat brain. Also, the mentioned protocol is a simple and efficient primary cell culture technique. The whole-brain was isolated from the skull and the meninges were removed carefully. Afterward, the cerebral hemispheres were mechanically and enzymatically digested. Then, the cell suspension was seeded in T25 culture flask and was incubated at 37 °C in the CO
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