Evidence map›Paper›PMID 31989326›Full record

ArticleJournal of industrial microbiology & biotechnology2020

Construction of a switchable synthetic Escherichia coli for aromatic amino acids by a tunable switch.

Xiaozhen Liu, Hao Niu, Zhaosong Huang, Qiang Li, Pengfei Gu

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of industrial microbiology & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Xiaozhen LiuSchool of Biological Science and Technology, University of Jinan, Jinan, 250022, People's Republic of China.
Hao NiuSchool of Biological Science and Technology, University of Jinan, Jinan, 250022, People's Republic of China.
Zhaosong HuangSchool of Biological Science and Technology, University of Jinan, Jinan, 250022, People's Republic of China.
Qiang LiSchool of Biological Science and Technology, University of Jinan, Jinan, 250022, People's Republic of China.
Pengfei GuSchool of Biological Science and Technology, University of Jinan, Jinan, 250022, People's Republic of China. bio_gupf@ujn.edu.cn.
University of Jinan · CN

Funding

National Natural Science Foundation of China 31600066National Natural Science Foundation of China 31741007National Natural Science Foundation of China 31870105Natural Science Foundation of Shandong Province ZR2016CB20Natural Science Foundation of Shandong Province ZR2016CL02State Key Laboratory of Microbial Technology Open Projects M2016-10
6 · The paper itself

Abstract

Escherichia coli, a model microorganism for which convenient metabolic engineering tools are available and that grows quickly in cheap media, has been widely used in the production of valuable chemicals, including aromatic amino acids. As the three aromatic amino acids, L-tryptophan, L-tyrosine, and L-phenylalanine, share the same precursors, to increase the titer of a specific aromatic amino acid, the branch pathways to the others are usually permanently inactivated, which leads to the generation of auxotrophic strains. In this study, a tunable switch that can toggle between different states was constructed. Then, a switchable and non-auxotrophic E. coli strain for synthesis of aromatic amino acids was constructed using this tunable switch. By adding different inducers to cultures, three different production patterns of aromatic amino acids by the engineered strain could be observed. This tunable switch can also be applied in regulating other branch pathways and in other bacteria.

Indexed as

Escherichia coliMetabolic EngineeringPhenylalanineTryptophanTyrosinePhenylalanineTryptophanTyrosineAromatic amino acidsEscherichia coliSwitchable synthetic strainTunable switch

Identifiers

PMID31989326
OpenAlexW3003614703

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.