Evidence map›Paper›PMID 31989196›Full record

ArticleAnalytical and bioanalytical chemistry2020

Detection of E. coli labeled with metal-conjugated antibodies using lateral-flow assay and laser-induced breakdown spectroscopy.

Carmen Gondhalekar, Eva Biela, Bartek Rajwa, Euiwon Bae, Valery Patsekin, Jennifer Sturgis, Cole Reynolds, Iyll-Joon Doh, Prasoon Diwakar, Larry Stanker and 4 more

Abstract read
PubMed Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.8field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Review
  3. Use of Lateral Flow Assays in Forensics.Sensors (Basel, Switzerland) · 2023
    Review
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Carmen GondhalekarWeldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Eva BielaDepartment of Basic Medical Sciences, Purdue University, West Lafayette, IN, 47907, USA.
Bartek RajwaBindley Bioscience Center, Discovery Park, Purdue University, West Lafayette, IN, 47907, USA.
Euiwon BaeSchool of Mechanical Engineering, Applied Optics Laboratory, Purdue University, West Lafayette, IN, 47907, USA.
Valery PatsekinDepartment of Basic Medical Sciences, Purdue University, West Lafayette, IN, 47907, USA.
Jennifer SturgisDepartment of Basic Medical Sciences, Purdue University, West Lafayette, IN, 47907, USA.
Cole ReynoldsWeldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Iyll-Joon DohSchool of Mechanical Engineering, Applied Optics Laboratory, Purdue University, West Lafayette, IN, 47907, USA.
Prasoon DiwakarMechanical Engineering Department, South Dakota School of Mines & Technology, Rapid City, SD, 57701, USA.
Larry StankerUnited States Department of Agriculture, USDA Research Services, Albany, CA, 94706, USA.
Vassilia ZorbaEnergy Technologies Area, Lawrence Berkeley National Laboratories, Berkeley, CA, 94720, USA.
Xianglei MaoEnergy Technologies Area, Lawrence Berkeley National Laboratories, Berkeley, CA, 94720, USA.
Richard RussoEnergy Technologies Area, Lawrence Berkeley National Laboratories, Berkeley, CA, 94720, USA.
J Paul RobinsonWeldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA. jpr@flowcyt.cyto.purdue.edu.
Purdue University West Lafayette · USLawrence Berkeley National Laboratory · USSouth Dakota School of Mines and Technology · USUnited States Department of Agriculture · US

Funding

Food Safety and Inspection Service 1935-42000-072-02GNational Science Foundation DGE-1333468Office of Defense Nuclear Nonproliferation DE-AC02-05CH11231
6 · The paper itself

Abstract

This study explores the adoption of laser-induced breakdown spectroscopy (LIBS) for the analysis of lateral-flow immunoassays (LFIAs). Gold (Au) nanoparticles are standard biomolecular labels among LFIAs, typically detected via colorimetric means. A wide diversity of lanthanide-complexed polymers (LCPs) are also used as immunoassay labels but are inapt for LFIAs due to lab-bound detection instrumentation. This is the first study to show the capability of LIBS to transition LCPs into the realm of LFIAs, and one of the few to apply LIBS to biomolecular label detection in complete immunoassays. Initially, an in-house LIBS system was optimized to detect an Au standard through a process of line selection across acquisition delay times, followed by determining limit of detection (LOD). The optimized LIBS system was applied to Au-labeled Escherichia coli detection on a commercial LFIA; comparison with colorimetric detection yielded similar LODs (1.03E4 and 8.890E3 CFU/mL respectively). Optimization was repeated with lanthanide standards to determine if they were viable alternatives to Au labels. It was found that europium (Eu) and ytterbium (Yb) may be more favorable biomolecular labels than Au. To test whether Eu-complexed polymers conjugated to antibodies could be used as labels in LFIAs, the conjugates were successfully applied to E. coli detection in a modified commercial LFIA. The results suggest interesting opportunities for creating highly multiplexed LFIAs. Multiplexed, sensitive, portable, and rapid LIBS detection of biomolecules concentrated and labeled on LFIAs is highly relevant for applications like food safety, where in-field food contaminant detection is critical. Graphical abstract.

Indexed as

LasersAntibodies, BacterialEscherichia coliMetalsSpectrum AnalysisAntibodies, BacterialMetalsE. coliLanthanidesLaser-induced breakdown spectroscopyLateral-flow immunoassayMetal-conjugated antibodies

Identifiers

PMID31989196
OpenAlexW3001886353

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.