Evidence map›Paper›PMID 31989002›Full record

ArticleData in brief2020

Data describing the experimental design and quality control of RNA-Seq of human adipose-derived stem cells undergoing early adipogenesis and osteogenesis.

Bruna H Marcon, Lucia Spangenberg, Bernardo Bonilauri, Anny Waloski Robert, Addeli Bez Batti Angulski, Guillermo Cabrera Cabo, Axel R Cofré, Paulo Sergio Loiacono Bettes, Bruno Dallagiovanna, Patrícia Shigunov

Open access · goldAbstract read
In one paragraph

Article in Data in brief, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Bruna H MarconLaboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil.
Lucia SpangenbergUnidad de Bioinformática, Institut Pasteur Montevideo, Mataojo 2020, Montevideo, 11400, Uruguay.
Bernardo BonilauriLaboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil.
Anny Waloski RobertLaboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil.
Addeli Bez Batti AngulskiLaboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil.
Guillermo Cabrera CaboUnidad de Bioinformática, Institut Pasteur Montevideo, Mataojo 2020, Montevideo, 11400, Uruguay.
Axel R CofréLaboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil.
Paulo Sergio Loiacono BettesCirurgia Plástica Dr. Paulo Bettes, Rua Francisco Rocha, 1312, Curitiba, PR, 80730-390, Brazil.
Bruno DallagiovannaLaboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil.
Patrícia ShigunovLaboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil.
Fundação Carlos Chagas · BRInstitut Pasteur de Montevideo · UYSociedade Brasileira de Cirurgia Plástica · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An important tool to study the regulation of gene expression is the sequencing and the analysis of different RNA fractions: total, ribosome-free, monosomal and polysomal. By comparing these different populations, it is possible to identity which genes are differentially expressed and to get information on how transcriptional and translational regulation modulates cellular function. Therefore, we used this strategy to analyze the regulation of gene expression of human adipose-derived stem cells during the triggering of the adipogenic and osteogenic differentiation. Here, we have focused on analyzing the differential expression of mRNAs during early adipogenic and osteogenic differentiation, and presented the detailed data concerning the experimental design, the RNA-Seq quality data, the raw data obtained and the RT-qPCR validation data. This information is important to confirm the accuracy of the data considering a future reuse of the data provided. Moreover, this study may be used as groundwork for future characterization of the transcriptome and the translatome regulation of different cell types.

Indexed as

AdipogenesisHuman adipose-derived stem cellsOsteogenesisPolysome profilingRNA-Seq

Identifiers

PMID31989002
PMCPMC6970145
OpenAlexW2997962613

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.