Evidence map›Paper›PMID 31988991›Full record

ArticleRegenerative therapy2020

Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers.

Denise Zujur, Kosuke Kanke, Shoko Onodera, Shoichiro Tani, Jenny Lai, Toshifumi Azuma, Xiaonan Xin, Alexander C Lichtler, David W Rowe, Taku Saito and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Regenerative therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 18 citations in OpenAlex.

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  13. The Progress of Stem Cell Technology for Skeletal Regeneration.International journal of molecular sciences · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 2 countries.

Denise ZujurDepartment of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Kosuke KankeDepartment of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Shoko OnoderaDepartment of Biochemistry, Tokyo Dental College, Tokyo, Japan.
Shoichiro TaniDepartment of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Jenny LaiDepartment of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Toshifumi AzumaDepartment of Biochemistry, Tokyo Dental College, Tokyo, Japan.
Xiaonan XinDepartment of Reconstructive Sciences, University of Connecticut Health Center, Farmington, CT, USA.
Alexander C LichtlerDepartment of Reconstructive Sciences, University of Connecticut Health Center, Farmington, CT, USA.
David W RoweDepartment of Reconstructive Sciences, University of Connecticut Health Center, Farmington, CT, USA.
Taku SaitoDepartment of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Sakae TanakaDepartment of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Hideki MasakiCenter for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Hiromitsu NakauchiCenter for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Ung-Il ChungDepartment of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Hironori HojoDepartment of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Shinsuke OhbaDepartment of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
The University of Tokyo · JPUConn Health · USTokyo Dental College · JP

Funding

Skeletal Phenotyping of Heterozygotes from IMPC Embryonic Lethal LinesR01HD098636 · NICHD · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI MAYE, PETER, ROWE, DAVID W. · 2019 to 2023
$2.8M
Adult Stem Cells: Osteoblast Differentiation and EngraftmentR01AR052374 · NIAMS · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI ROWE, DAVID W. · 2007 to 2011
$1.5M
NIAMS NIH HHS R01 AR052374NICHD NIH HHS R01 HD098636
6 · The paper itself

Abstract

Clinically relevant human induced pluripotent stem cell (hiPSC) derivatives require efficient protocols to differentiate hiPSCs into specific lineages. Here we developed a fully defined xeno-free strategy to direct hiPSCs toward osteoblasts within 21 days. The strategy successfully achieved the osteogenic induction of four independently derived hiPSC lines by a sequential use of combinations of small-molecule inducers. The induction first generated mesodermal cells, which subsequently recapitulated the developmental expression pattern of major osteoblast genes and proteins. Importantly, Col2.3-Cherry hiPSCs subjected to this strategy strongly expressed the cherry fluorescence that has been observed in bone-forming osteoblasts

Indexed as

HedgehogHelioxanthinMesodermThree-dimensional cultureWnt

Identifiers

PMID31988991
PMCPMC6965656
OpenAlexW2999131420

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.