Evidence map›Paper›PMID 31988458›Full record

ArticleNature biomedical engineering2020

Robotic fluidic coupling and interrogation of multiple vascularized organ chips.

Richard Novak, Miles Ingram, Susan Marquez, Debarun Das, Aaron Delahanty, Anna Herland, Ben M Maoz, Sauveur S F Jeanty, Mahadevabharath R Somayaji, Morgan Burt and 47 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature biomedical engineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 196 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
196citing papers in PubMed, 2 pooled it
22.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

196 citing papers in PubMed, 2 syntheses or guidelines pooled it, 390 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Review
  5. Review
  6. Review
  7. Review
  8. Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. Review
  14. Review
  15. Article
  16. Review
  17. Article
  18. Review
  19. Article
  20. Review

136 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

57 authors at 3 institutions in 5 countries.

Richard Novak *Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0003-0485-4284
Miles IngramWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Susan MarquezWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0003-1183-8522
Debarun Das *CFD Research Corporation, Huntsville, AL, USA.
Aaron DelahantyWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0001-5206-4880
Anna Herland *Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0002-5002-2537
Ben M Maoz *Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0002-3823-7682
Sauveur S F JeantyWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Mahadevabharath R Somayaji *CFD Research Corporation, Huntsville, AL, USA.
Morgan BurtWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Elizabeth CalamariWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Angeliki ChalkiadakiWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Alexander ChoCFD Research Corporation, Huntsville, AL, USA.
Youngjae ChoeWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
David Benson ChouWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0002-1659-9308
Michael CronceWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Stephanie DauthWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Toni DivicWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Jose Fernandez-AlconWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Thomas FerranteWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
John FerrierWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0003-2489-1066
Edward A FitzGeraldWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0002-0603-1241
Rachel FlemingWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Sasan Jalili-FiroozinezhadWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0002-5004-893X
Thomas GrevesseWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Josue A GossWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Tiama Hamkins-IndikWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Olivier HenryWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Chris HinojosaWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Tessa HuffstaterWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Kyung-Jin JangWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0001-7872-1703
Ville KujalaWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Lian LengWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Robert MannixWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Yuka MiltonWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Janna NawrothWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Bret A NestorWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Carlos F NgWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Blakely O'ConnorWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Tae-Eun ParkWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0003-3979-5405
Henry SanchezWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Josiah SlizWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Alexandra Sontheimer-PhelpsWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0003-4732-2835
Ben SwenorWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0002-4394-5077
Guy ThompsonWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
George J TouloumesWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Zachary TranchemontagneWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Norman WenWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Moran YadidWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Anthony BahinskiWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Geraldine A HamiltonWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Daniel LevnerWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Oren LevyWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Andrzej PrzekwasCFD Research Corporation, Huntsville, AL, USA.
Rachelle Prantil-Baun *Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Kevin K ParkerWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Donald E IngberWyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA. don.ingber@wyss.harvard.edu.ORCID http://orcid.org/0000-0002-4319-6520
Harvard University · USCFD Research Corporation (United States) · USBoston Children's Hospital · US

Funding

MOLECULAR IMMUNOLOGY AND TUMOR BIOLOGYT32CA009216 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI David Michael Langenau · 1985 to 2026
$10.6M
NCI NIH HHS T32 CA009216
6 · The paper itself

Abstract

Organ chips can recapitulate organ-level (patho)physiology, yet pharmacokinetic and pharmacodynamic analyses require multi-organ systems linked by vascular perfusion. Here, we describe an 'interrogator' that employs liquid-handling robotics, custom software and an integrated mobile microscope for the automated culture, perfusion, medium addition, fluidic linking, sample collection and in situ microscopy imaging of up to ten organ chips inside a standard tissue-culture incubator. The robotic interrogator maintained the viability and organ-specific functions of eight vascularized, two-channel organ chips (intestine, liver, kidney, heart, lung, skin, blood-brain barrier and brain) for 3 weeks in culture when intermittently fluidically coupled via a common blood substitute through their reservoirs of medium and endothelium-lined vascular channels. We used the robotic interrogator and a physiological multicompartmental reduced-order model of the experimental system to quantitatively predict the distribution of an inulin tracer perfused through the multi-organ human-body-on-chips. The automated culture system enables the imaging of cells in the organ chips and the repeated sampling of both the vascular and interstitial compartments without compromising fluidic coupling.

Indexed as

Lab-On-A-Chip DevicesBlood-Brain BarrierBrainCalibrationCell Culture TechniquesEquipment DesignHeartHumansIntestinesKidneyLiverLungMicrofluidicsRoboticsSkin

Identifiers

PMID31988458
PMCPMC8057865
OpenAlexW3002045056

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.