ArticleScientific reports2020
Shotgun proteomics, in-silico evaluation and immunoblotting assays for allergenicity assessment of lesser mealworm, black soldier fly and their protein hydrolysates.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 66 citations in OpenAlex.
- Allergenicity assessment of novel foods - lessons from immune endotyping of food-allergic patients.Frontiers in immunology · 2026Review
- Sustainable Emerging Proteins: Allergenic Proteins in Edible Insects, Microalgae, and Microorganisms, and Desensitization Processing Technologies.Foods (Basel, Switzerland) · 2025Review
- Edible Insects and Allergenic Potential: An Observational Study About In Vitro IgE-Reactivity to Recombinant Pan-Allergens of the Black Soldier Fly (International journal of molecular sciences · 2025Observational
- Edible Insects as Future Proteins: Nutritional Value, Functional Properties, Bioactivities, and Safety Perspectives.Nutrients · 2025Review
- Chemopreventive Effects of Bioactive Peptides Derived from Black Soldier Fly Larvae Protein Hydrolysates in a Rat Model of Early-Stage Colorectal Carcinogenesis.International journal of molecular sciences · 2025Article
- Insects as Source of Nutraceuticals with Antioxidant, Antihypertensive, and Antidiabetic Properties: Focus on the Species Approved in Europe up to 2024.Foods (Basel, Switzerland) · 2025Review
- Edible Insects as an Alternative Source of Nutrients: Benefits, Risks, and the Future of Entomophagy in Europe-A Narrative Review.Foods (Basel, Switzerland) · 2025Review
- Protein hydrolysates fromAnimal cells and systems · 2025Article
- Chemical and Microbiological Characterization of Freeze-Dried Superworm (Molecules (Basel, Switzerland) · 2024Article
- An Assessment of the Impact of Insect Meal in Dry Food on a Dog with a Food Allergy: A Case Report.Animals : an open access journal from MDPI · 2024Article
- Unravelling the Influence of Extraction Techniques on Protein Yield and Nutritional Value in Lesser Mealworm Larvae.Molecules (Basel, Switzerland) · 2024Article
- The Effect of Dietary Protein Hydrolysate from Black Soldier Fly Larvae and Schizochytrium on Palatability, Nutrient Metabolites and Health Status in Beagle Dogs.Metabolites · 2024Article
- An analysis of emerging food safety and fraud risks of novel insect proteins within complex supply chains.NPJ science of food · 2024Review
- Effect of Pulsed Electric Field Technology on the Composition and Bioactive Compounds of Black Soldier Fly Larvae Dried with Convective and Infrared-Convective Methods.Molecules (Basel, Switzerland) · 2023Article
- Advanced Proteomic and Bioinformatic Tools for Predictive Analysis of Allergens in Novel Foods.Biology · 2023Review
- Cytoprotective and Antioxidant Effects of Hydrolysates from Black Soldier Fly (Antioxidants (Basel, Switzerland) · 2023Article
- Locust protein hydrolysates have the potential to enhance the storage stability of cheese.Current research in food science · 2023Article
- Nutritional Composition, Health Benefits, and Application Value of Edible Insects: A Review.Foods (Basel, Switzerland) · 2022Review
- Effectiveness of enzymatic hydrolysis for reducing the allergenic potential of legume by-products.Scientific reports · 2022Article
- Safety of frozen and freeze-dried formulations of the lesser mealworm (EFSA journal. European Food Safety Authority · 2022Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since 2018, insects have belonged the category of Novel Foods and the presence of allergens represents one of the main hazards connected to their consumption, also due to the potential cross-reactivity with Arthropoda pan-allergens. In the present work, the allergenicity assessment of black soldier fly and lesser mealworm was performed with a shotgun bottom-up proteomic approach combined with in-silico assessment, followed by IgG- and IgE-immunoblotting experiments. The peptides identified, filtered for their abundance and robustness, belonged mainly to muscle proteins, which represented the most abundant protein group. The relevant potential allergens were in-silico identified by sequence similarity to known allergens, and among them tropomyosin resulted the most abundant insect allergen. IgG-immunoblotting analysis with anti-Tropomyosin I antibodies and IgE-immunoblotting assay with serum from patient allergic to crustacean tropomyosin were performed in order to assess the immunoreactivity in both insects. The immunoassays were carried out also on protein hydrolysates extracted by treating insects with Protease from Bacillus licheniformis (1%, 60 °C, pH 7.5). While IgG-immunoblotting demonstrated the loss of immunoreactivity for both hydrolysates, IgE-immunoblotting showed a partial immunoreactivity preservation, also after hydrolysis, in the case of black soldier fly hydrolysate, and a total loss of immunoreactivity for lesser mealworm hydrolysate.
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