ArticleThe Journal of cell biology1988
Isolation and characterization of the mouse acetylcholine receptor delta subunit gene: identification of a 148-bp cis-acting region that confers myotube-specific expression.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 65 citations in OpenAlex.
- Neu differentiation factor stimulates phosphorylation and activation of the Sp1 transcription factor.Molecular and cellular biology · 1999Article
- Implication of a multisubunit Ets-related transcription factor in synaptic expression of the nicotinic acetylcholine receptor.The EMBO journal · 1998Article
- Identification of a DNA element determining synaptic expression of the mouse acetylcholine receptor delta-subunit gene.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- The basic helix-loop-helix protein upstream stimulating factor regulates the cardiac ventricular myosin light-chain 2 gene via independent cis regulatory elements.Molecular and cellular biology · 1994Article
- Rapid inhibition of myogenin-driven acetylcholine receptor subunit gene transcription.The EMBO journal · 1994Article
- An E box mediates activation and repression of the acetylcholine receptor delta-subunit gene during myogenesis.Molecular and cellular biology · 1993Article
- An 83-nucleotide promoter of the acetylcholine receptor epsilon-subunit gene confers preferential synaptic expression in mouse muscle.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Electrical activity-dependent regulation of the acetylcholine receptor delta-subunit gene, MyoD, and myogenin in primary myotubes.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Analysis of binding and activating functions of the chick muscle acetylcholine receptor gamma-subunit upstream sequence.Cellular and molecular neurobiology · 1992Article
- The 5'-flanking region of the mouse muscle nicotinic acetylcholine receptor beta subunit gene promotes expression in cultured muscle cells and is activated by MRF4, myogenin and myoD.Nucleic acids research · 1992Article
- Different mechanisms regulate muscle-specific AChR gamma- and epsilon-subunit gene expression.The EMBO journal · 1991Article
- Rabbit muscle creatine kinase: genomic cloning, sequencing, and analysis of upstream sequences important for expression in myocytes.Nucleic acids research · 1991Article
- Tissue-specific transcription of the cardiac myosin light-chain 2 gene is regulated by an upstream repressor element.Molecular and cellular biology · 1991Article
- Expression of the acetylcholine receptor delta-subunit gene in differentiating chick muscle cells is activated by an element that contains two 16 bp copies of a segment of the alpha-subunit enhancer.The EMBO journal · 1990Article
- A new myocyte-specific enhancer-binding factor that recognizes a conserved element associated with multiple muscle-specific genes.Molecular and cellular biology · 1989Article
- Identification of a myocyte nuclear factor that binds to the muscle-specific enhancer of the mouse muscle creatine kinase gene.Molecular and cellular biology · 1989Article
- Ligand-gated ion channels. Homology and diversity.Molecular neurobiologyReview
- Activity-dependent regulation of gene expression in muscle and neuronal cells.Molecular neurobiologyReview
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have isolated the gene encoding the delta subunit of the mouse skeletal muscle acetylcholine receptor (AChR) and have identified a 148-bp cis-acting region that controls cell type-specific and differentiation-dependent gene expression. The 5' flanking region of the delta subunit gene was fused to the protein-coding region of the chloramphenicol acetyltransferase (CAT) gene and gene fusions were transfected into C2 mouse skeletal muscle cells. Both transiently and stably transfected cells were assayed for CAT gene expression. Deletions from the 5' end of the mouse delta gene demonstrate that 148 bp of 5' flanking DNA is sufficient to confer cell type-specific and differentiation-dependent expression: CAT activity is present in transfected myotubes, but not in transfected 3T3 cells or 10T1/2 cells. Moreover, the level of CAT expression in myotubes transfected with constructs containing 148 bp of 5' flanking DNA from the delta subunit gene is identical to that in myotubes transfected with constructs containing 3.2 kb of 5' flanking DNA and similar to expression from the SV-40 early promoter. Increased CAT activity in myotubes is a result of an increased rate of transcription from the delta subunit promoter, since CAT RNA levels are also 35-fold more abundant in myotubes than myoblasts. In contrast, the SV-40 early promoter is similarly active in all cell types. Thus, 148 bp of 5' flanking DNA from the delta subunit gene contains all the information required for cell type-specific and differentiation-dependent expression of the AChR delta subunit.
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