ArticleThe Journal of cell biology1988
Real-time observations of microtubule dynamic instability in living cells.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
110 citing papers in PubMed, 368 citations in OpenAlex.
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- Mechanical coupling coordinates microtubule growth.bioRxiv : the preprint server for biology · 2023Article
- Tau, microtubule dynamics, and axonal transport: New paradigms for neurodegenerative disease.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Article
- A Comprehensive Study on the Electrostatic Properties of Tubulin-Tubulin Complexes in Microtubules.Cells · 2023Article
- Stochastic model of T cell repolarization during target elimination (II).Biophysical journal · 2022Article
- A celebration of the 25th anniversary of chromatin-mediated spindle assembly.Molecular biology of the cell · 2022Article
- TH588 and Low-Dose Nocodazole Impair Chromosome Congression by Suppressing Microtubule Turnover within the Mitotic Spindle.Cancers · 2021Article
- Mechanics and kinetics of dynamic instability.eLife · 2020Article
- The quantification and regulation of microtubule dynamics in the mitotic spindle.Current opinion in cell biology · 2019Review
- Behavior of α, β tubulin in DMSO-containing electrolytes.Nanoscale advances · 2019Article
- Direct observation of branching MT nucleation in living animal cells.The Journal of cell biology · 2019Article
- Intracellular mechanisms of fungal space searching in microenvironments.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Microtubule Plus End Dynamics - Do We Know How Microtubules Grow?: Cells boost microtubule growth by promoting distinct structural transitions at growing microtubule ends.BioEssays : news and reviews in molecular, cellular and developmental biology · 2019Article
- Measuring microtubule dynamics.Essays in biochemistry · 2018Review
- Regulation of microtubule dynamic instability by the carboxy-terminal tail of β-tubulin.Life science alliance · 2018Article
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- Steady-state EB cap size fluctuations are determined by stochastic microtubule growth and maturation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Cross-hierarchy systems principles.Current opinion in systems biology · 2017Article
50 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Individual microtubule dynamics were observed in real time in primary cultures of newt lung epithelium using video-enhanced differential interference contrast microscopy and digital image processing. The linear filaments observed in cells corresponded to microtubules based on three criteria: (a) small particles translocated along them; (b) the majority of them disappeared after incubation in nocodazole; (c) and the distribution observed by differential interference contrast correlated with anti-tubulin immunofluorescence staining of the same cell. Microtubules were most clearly observed at the leading edge of cells located at the periphery of the epithelial sheet. Microtubules exhibited dynamic instability behavior: individual microtubules existed in persistent phases of elongation or rapid shortening. Microtubules elongated at a velocity of 7.2 micron/min +/- 0.3 SEM (n = 42) and rapidly shortened at a velocity of 17.3 micron/min +/- 0.7 SEM (n = 35). The transitions between elongation and rapid shortening occurred abruptly and stochastically with a transition frequency of 0.014 s-1 for catastrophe and 0.044 s-1 for rescue. Approximately 70% of the rapidly shortening microtubules were rescued and resumed elongation within the 35 x 35 micron microscopic field. A portion of the microtubule population appeared differentially stable and did not display any measurable elongation or shortening during 10-15-min observations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.