ArticlePloS one2020
Affective state determination in a mouse model of colitis-associated colorectal cancer.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- "Do not look at me like that": Is the facial expression score reliable and accurate to evaluate pain in large domestic animals? A systematic review.Frontiers in veterinary science · 2022Pooled it
- Molecular and immune profiling of colorectal polyps using FTIR microspectroscopy.Molecular and cellular biochemistry · 2026Article
- Evaluating the analgesic effect of metamizole in the mouse model for DSS-induced acute colitis.Frontiers in physiology · 2026Article
- OBSERVE: guidelines for the refinement of rodent cancer models.Nature protocols · 2024Review
- The grimace scale: a useful tool for assessing pain in laboratory animals.Experimental animals · 2024Review
- Review of Rat (Animals : an open access journal from MDPI · 2023Review
- Grimace scale assessment duringFrontiers in veterinary science · 2023Article
- Ecological and dynamic analysis of gut microbiota in the early stage of azomethane-dextran sodium sulfate model in mice.Frontiers in cellular and infection microbiology · 2023Article
- Chemically Induced Colitis-Associated Cancer Models in Rodents for Pharmacological Modulation: A Systematic Review.Journal of clinical medicine · 2022Review
- Antinociceptive Effects of Kappa-Opioid Receptor Agonists.Handbook of experimental pharmacology · 2022Review
- The neurobiology of pain and facial movements in rodents: Clinical applications and current research.Frontiers in veterinary science · 2022Review
- Induction of colorectal carcinogenesis in the C57BL/6J and A/J mouse strains with a reduced DSS dose in the AOM/DSS model.Laboratory animal research · 2021Article
- Diagnostic Ability of Methods Depicting Distress of Tumor-Bearing Mice.Animals : an open access journal from MDPI · 2021Article
- Methods Used and Application of the Mouse Grimace Scale in Biomedical Research 10 Years on: A Scoping Review.Animals : an open access journal from MDPI · 2021Article
- The Utility of Grimace Scales for Practical Pain Assessment in Laboratory Animals.Animals : an open access journal from MDPI · 2020Review
- Animal Models of Cancer-Related Pain: Current Perspectives in Translation.Frontiers in pharmacology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Behavioural indicators of affective state, including burrowing, clinical scores and the Mouse Grimace Score have not yet been validated in mouse models of chronic gastrointestinal disease. Additionally, a comparison of these methods has not been characterised. This study aimed to determine which behavioural assessment was the optimal indicator of disease, evidenced by correlation with clinically-assessed measures, in an azoxymethane (AOM)/dextran sulphate sodium (DSS) mouse model of colitis-associated colorectal cancer. C57BL/6 mice were allocated to four groups (n = 10/group); 1) saline control, 2) saline+buprenorphine, 3) AOM+DSS+water, 4) AOM+DSS+buprenorphine. Mice were gavaged thrice weekly with water or buprenorphine (0.5mg/kg; 80μL) for 9 weeks. Disease activity index (DAI) was measured daily; burrowing and grimace analyses occurred on days -1, 5, 19, 26, 40, 47 and 61. Colonoscopies were performed on days 20, 41 and 62. All animals were euthanized on day 63. Burrowing activity and retrospective grimace analyses were unaffected (P>0.05), whilst DAI was significantly increased (P<0.05) in mice with colitis-associated colorectal cancer compared to normal controls. In addition, DAI was positively correlated with colonoscopically-assessed severity and tumour number (P<0.05). We conclude that traditional measures of DAI or clinical scoring provide the most reliable assessment of wellbeing in mice with colitis-associated colorectal cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.