Evidence map›Paper›PMID 31986159›Full record

ArticlePloS one2020

Increased mobilization of mesenchymal stem cells in patients with acute respiratory distress syndrome undergoing extracorporeal membrane oxygenation.

Christian Patry, Thalia Doniga, Franziska Lenz, Tim Viergutz, Christel Weiss, Burkhard Tönshoff, Armin Kalenka, Benito Yard, Jörg Krebs, Thomas Schaible and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
1.1field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 16 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Christian PatryDepartment of Pediatrics I, University Children's Hospital Heidelberg, University of Heidelberg, Heidelberg, Germany.ORCID 0000-0002-1270-6508
Thalia DonigaDepartment of Neonatology, University Children's Hospital Mannheim, University of Heidelberg, Heidelberg, Germany.
Franziska LenzDepartment of Anaesthesiology and Intensive Care Medicine, University Medical Center Mannheim, University of Heidelberg, Heidelberg, Germany.
Tim ViergutzDepartment of Anaesthesiology and Intensive Care Medicine, University Medical Center Mannheim, University of Heidelberg, Heidelberg, Germany.
Christel WeissDepartment of Medical Statistics and Biomathematics, Medical Faculty Mannheim, University of Heidelberg, Heidelberg, Germany.
Burkhard TönshoffDepartment of Pediatrics I, University Children's Hospital Heidelberg, University of Heidelberg, Heidelberg, Germany.
Armin KalenkaDepartment of Anaesthesiology and Intensive Care Medicine, University Medical Center Mannheim, University of Heidelberg, Heidelberg, Germany.
Benito YardDepartment of Medicine V, University Medical Center Mannheim, University of Heidelberg, Heidelberg, Germany.
Jörg KrebsDepartment of Anaesthesiology and Intensive Care Medicine, University Medical Center Mannheim, University of Heidelberg, Heidelberg, Germany.
Thomas SchaibleDepartment of Neonatology, University Children's Hospital Mannheim, University of Heidelberg, Heidelberg, Germany.
Grietje BeckDepartment of Anaesthesiology and Intensive Care Medicine, Dr. Horst-Schmidt Clinic, Wiesbaden, Germany.
Neysan RafatDepartment of Pediatrics I, University Children's Hospital Heidelberg, University of Heidelberg, Heidelberg, Germany.
Heidelberg University · DEHelios Dr. Horst Schmidt Kliniken Wiesbaden · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe acute respiratory distress syndrome (ARDS) is characterized by pulmonary epithelial and endothelial barrier dysfunction and injury. In severe forms of ARDS, extracorporeal membrane oxygenation (ECMO) is often the last option for life support. Endothelial progenitor (EPC) and mesenchymal stem cells (MSC) can regenerate damaged endothelium and thereby improve pulmonary endothelial dysfunction. However, we still lack sufficient knowledge about how ECMO might affect EPC- and MSC-mediated regenerative pathways in ARDS. Therefore, we investigated if ECMO impacts EPC and MSC numbers in ARDS patients.

methodsPeripheral blood mononuclear cells from ARDS patients undergoing ECMO (n = 16) and without ECMO support (n = 12) and from healthy volunteers (n = 16) were isolated. The number and presence of circulating EPC and MSC was detected by flow cytometry. Serum concentrations of vascular endothelial growth factor (VEGF) and angiopoietin 2 (Ang2) were determined.

resultsIn the ECMO group, MSC subpopulations were higher by 71% compared to the non-ECMO group. Numbers of circulating EPC were not significantly altered. During ECMO, VEGF and Ang2 serum levels remained unchanged compared to the non-ECMO group (p = 0.16), but Ang2 serum levels in non-survivors of ARDS were significantly increased by 100% (p = 0.02) compared to survivors.

conclusionsECMO support in ARDS is specifically associated with an increased number of circulating MSC, most likely due to enhanced mobilization, but not with a higher numbers of EPC or serum concentrations of VEGF and Ang2.

Indexed as

Extracorporeal Membrane OxygenationAdultAngiopoietin-2Case-Control StudiesEndothelial Progenitor CellsFemaleHumansLeukocytes, MononuclearMaleMesenchymal Stem CellsMiddle AgedRespiratory Distress SyndromeVascular Endothelial Growth Factor AAngiopoietin-2Vascular Endothelial Growth Factor A

Identifiers

PMID31986159
PMCPMC6984734
OpenAlexW3001691593

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.