Evidence map›Paper›PMID 31986129›Full record

ArticlePLoS biology2020

Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult Drosophila.

Leslie Ann Jaramillo Koyama, Andrés Aranda-Díaz, Yu-Han Su, Shruthi Balachandra, Judy Lisette Martin, William B Ludington, Kerwyn Casey Huang, Lucy Erin O'Brien

Abstract read
In one paragraph

Article in PLoS biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Spatial microenvironments tune immune response dynamics in thebioRxiv : the preprint server for biology · 2025
    Article
  7. Review
  8. Article
  9. Drosophila Intestine as a Model to Study Tumors.Advances in experimental medicine and biology · 2025
    Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Review
  20. Cells · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Leslie Ann Jaramillo KoyamaDepartment of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, United States of America.ORCID 0000-0002-2179-9160
Andrés Aranda-DíazDepartment of Bioengineering, Stanford University, Stanford, California, United States of America.ORCID 0000-0002-0566-4901
Yu-Han SuDepartment of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, United States of America.ORCID 0000-0002-6035-9395
Shruthi BalachandraDepartment of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, United States of America.
Judy Lisette MartinDepartment of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, United States of America.
William B LudingtonDepartment of Embryology, Carnegie Institution of Washington, Baltimore, Maryland, United States of America.ORCID 0000-0001-9637-4493
Kerwyn Casey HuangDepartment of Bioengineering, Stanford University, Stanford, California, United States of America.ORCID 0000-0002-8043-8138
Lucy Erin O'BrienDepartment of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, United States of America.ORCID 0000-0001-7660-2524

Funding

Resource Component: Acquisition, maintenance and distribution of Drosophila stocksP40OD018537 · OD · TRUSTEES OF INDIANA UNIVERSITY · PI KEVIN R COOK · 2014 to 2026
$13.5M
GENETICS TRAINING PROGRAM FOR PHD CANDIDATEST32GM007790 · NIGMS · STANFORD UNIVERSITY · PI BRUNET, ANNE, KINGSLEY, DAVID M · 1985 to 2020
$12.8M
Developing a reduced complexity model gut microbiome in the behavior model, Drosophila melanogasterDP5OD017851 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI LUDINGTON, WILLIAM BASIL · 2013 to 2017
$2.0M
Dynamic Mechanisms of Fate Control during Epithelial Organ RenewalR01GM116000 · NIGMS · STANFORD UNIVERSITY · PI O'BRIEN, LUCY ERIN · 2016 to 2020
$1.6M
Nikon A1Rsi resonant spectral confocal microscopeS10OD010580 · OD · STANFORD UNIVERSITY · PI FULLER, MARGARET T · 2013 to 2013
$377k
Cell cycle regulation of fate outcomes in adult stem cells during adaptive organ growth and homeostasisF31GM123736 · NIGMS · STANFORD UNIVERSITY · PI KOYAMA, LESLIE ANN JARAMILLO · 2017 to 2018
$71k
Howard Hughes Medical InstituteNIGMS NIH HHS F31 GM123736NIGMS NIH HHS R01 GM116000NIGMS NIH HHS T32 GM007790NIH HHS DP5 OD017851NIH HHS P40 OD018537NIH HHS S10 OD010580
6 · The paper itself

Abstract

Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here, we describe Bellymount, a simple, noninvasive method for longitudinal imaging of the Drosophila abdomen at subcellular resolution. Bellymounted animals remain live and intact, so the same individual can be imaged serially to yield vivid time series of multiday processes. This feature opens the door to longitudinal studies of Drosophila internal organs in their native context. Exploiting Bellymount's capabilities, we track intestinal stem cell lineages and gut microbial colonization in single animals, revealing spatiotemporal dynamics undetectable by previously available methods.

Indexed as

Gastrointestinal MicrobiomeAge FactorsAnatomy, Cross-SectionalAnimalsDrosophilaIntestinesIntravital MicroscopyOptical ImagingViscera

Identifiers

PMID31986129
PMCPMC7004386

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.