ArticleBiology methods & protocols2020
Single-cell transcriptomics from human pancreatic islets: sample preparation matters.
Article in Biology methods & protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- SPICEY: an R package for quantifying tissue specificity from single cell multi-omics data.BMC bioinformatics · 2026Article
- Article
- Human pancreatic α-cell heterogeneity and trajectory inference analyses reveal SMOC1 as a β-cell dedifferentiation gene.Nature communications · 2025Article
- Latent plasticity of the human pancreas across development, health, and disease.bioRxiv : the preprint server for biology · 2025Article
- Approaching the holistic transcriptome-convolution and deconvolution in transcriptomics.Briefings in bioinformatics · 2025Review
- Considerations for building and using integrated single-cell atlases.Nature methods · 2025Review
- Single-cell transcriptomic profiling of human pancreatic islets reveals genes responsive to glucose exposure over 24 h.Diabetologia · 2024Article
- Ghrelin deletion and conditional ghrelin cell ablation increase pancreatic islet size in mice.The Journal of clinical investigation · 2023Article
- Functional interrogation of twenty type 2 diabetes-associated genes using isogenic human embryonic stem cell-derived β-like cells.Cell metabolism · 2023Article
- Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles.Genome medicine · 2023Article
- Review
- Gut mucosa dissociation protocols influence cell type proportions and single-cell gene expression levels.Scientific reports · 2022Article
- Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level.Journal of visualized experiments : JoVE · 2022Article
- Using single-nucleus RNA-sequencing to interrogate transcriptomic profiles of archived human pancreatic islets.Genome medicine · 2021Article
- Temporal single-cell regeneration studies: the greatest thing since sliced pancreas?Trends in endocrinology and metabolism: TEM · 2021Review
- Creation of a Single Cell RNASeq Meta-Atlas to Define Human Liver Immune Homeostasis.Frontiers in immunology · 2021Article
- Human Islets Contain a Beta Cell Type That Expresses Proinsulin But Not the Enzyme That Converts the Precursor to Insulin.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
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8 authors.
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Abstract
Single-cell RNA sequencing (scRNA-seq) of human primary tissues is a rapidly emerging tool for investigating human health and disease at the molecular level. However, optimal processing of solid tissues presents a number of technical and logistical challenges, especially for tissues that are only available at autopsy, which includes pancreatic islets, a tissue that is highly relevant to diabetes. To assess the possible effects of different sample preparation protocols on fresh islet samples, we performed a detailed comparison of scRNA-seq data generated with islets isolated from a human donor but processed according to four treatment strategies, including fixation and cryopreservation. We found significant and reproducible differences in the proportion of cell types identified, and more minor effects on cell-specific patterns of gene expression. Fresh islets from a second donor confirmed gene expression signatures of alpha and beta subclusters. These findings may well apply to other tissues, emphasizing the need for careful consideration when choosing processing methods, comparing results between different studies, and/or interpreting data in the context of multiple cell types from preserved tissue.
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