ArticleThe Journal of reproduction and development2020
Vitrification of porcine cumulus-oocyte complexes at the germinal vesicle stage does not trigger apoptosis in oocytes and early embryos, but activates anti-apoptotic Bcl-XL gene expression beyond the 4-cell stage.
Article in The Journal of reproduction and development, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 21 citations in OpenAlex.
- Induction of stress granules alleviates programmed cell death induced by lysosomal damage during NK cell cryopreservation.Cell death discovery · 2026Article
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- Optimization of bovine oocyte cryopreservation: membrane fusion competence and cell death of linoleic acid-Frontiers in veterinary science · 2025Article
- Ovarian expression of MerTK and its ligand Pros1 in non-pregnant estrus and pregnant mice.Journal of molecular histology · 2024Article
- New Approach to the Cryopreservation of GV Oocytes and Cumulus Cells through the Lens of Preserving the Intercellular Gap Junctions Based on the Bovine Model.International journal of molecular sciences · 2024Article
- Trehalose delivered by cold-responsive nanoparticles improves tolerance of cumulus-oocyte complexes to microwave drying.Journal of assisted reproduction and genetics · 2023Article
- Article
- Supplementation of SDF1 during Pig Oocyte In Vitro Maturation Improves Subsequent Embryo Development.Molecules (Basel, Switzerland) · 2022Article
- Vitrification of Pronuclear Zygotes Perturbs Porcine Zygotic Genome Activation.Animals : an open access journal from MDPI · 2022Article
- Effect of porcine immature oocyte vitrification on oocyte-cumulus cell gap junctional intercellular communication.Porcine health management · 2020Article
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Authors and funding
7 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The aim of the present study was to clarify whether or not our vitrification procedure at the germinal vesicle (GV)-stage triggers the apoptotic cascade in oocytes and subsequent embryos. Immature porcine cumulus-oocyte complexes were either vitrified and warmed (vitrified group) or subjected to cryoprotectant agents (CPA group) or cultured without any treatment (control). Oocytes of all treatment groups were subjected to in vitro maturation (IVM), fertilization, and embryo culture. Apoptosis was assayed in live oocytes at the end of IVM culture and in cleavage-stage embryos after in vitro fertilization (IVF). We detected similar frequencies of DNA fragmentation, levels of caspase activity, phosphatidylserine externalization, and mRNA levels for pro-apoptotic Bax and CASP3 genes in oocytes at the end of IVM and in early embryos among all groups. However, in the vitrified group, the anti-apoptotic Bcl-XL gene was upregulated in 4-8 cell embryos, which caused an 8-fold significant increase in the Bcl-XL/Bax mRNA ratio compared with the control and CPA groups (P < 0.05). In conclusion, vitrification of porcine oocytes at the GV stage by our method did not trigger the apoptotic cascade in oocytes and subsequent embryos but triggered the upregulation of the anti-apoptotic Bcl-XL gene in embryos.
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