ArticleCurrent biology : CB2020
Osmotic Gradients in Epithelial Acini Increase Mechanical Tension across E-cadherin, Drive Morphogenesis, and Maintain Homeostasis.
Article in Current biology : CB, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 73 citations in OpenAlex.
- Biophysics of lumen morphogenesis.Development (Cambridge, England) · 2026Review
- Apical-out polarity in epithelial spheroids requires α6β4 integrins, cell proliferation and anchorage independence.Journal of cell science · 2026Article
- Tubulogenesis of bovine uterine glands by epidermal growth factor and collagen I in 3D culture systems.Bioscience reports · 2026Article
- Construction, evaluation, and applications of renal barrier-on-a-chip system.Bioactive materials · 2026Review
- Permeability-driven pressure and cell proliferation control lumen morphogenesis in pancreatic organoids.Nature cell biology · 2026Article
- Extracellular Matrix Viscoelasticity Regulates Mammary Branching Morphogenesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- BEADS: An Interactive Semi-Automated Workflow for 3D Fibrin Angiogenesis Assays Enabling Co-Culture and Directionality Analysis.bioRxiv : the preprint server for biology · 2025Article
- Rotational migration in human pancreatic ductal organoids depends on actin and myosin activity.Communications biology · 2025Article
- Cell-mediated matrix deformations and cell-cell adhesions determine epithelial collective cell migration phenotypes.APL bioengineering · 2025Article
- Rotational migration in human pancreatic ductal organoids depends on actin and myosin activity.ArXiv · 2025Article
- Engineering Cellular Self-Adhesions Inside 3D Printed Micro-Arches to Enhance Cell:Biomaterial Attachment.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Fluid secretion and luminal pressure control lateral branching morphogenesis in the embryonic avian lung.Developmental biology · 2025Article
- Article
- The Role of the Swollen State in Cell Proliferation.The Journal of membrane biology · 2025Review
- Frontiers in mechanobiology and mechanomedicine.Med-X · 2025Article
- Mechanics of cell sheets: plectin as an integrator of cytoskeletal networks.Open biology · 2025Review
- Deciphering the interplay between biology and physics with a finite element method-implemented vertex organoid model: A tool for the mechanical analysis of cell behavior on a spherical organoid shell.PLoS computational biology · 2025Article
- Using Microfluidics to Align Matrix Architecture and Generate Chemokine Gradients Promotes Directional Branching in a Model of Epithelial Morphogenesis.ACS biomaterials science & engineering · 2024Article
- Annotation Vocabulary (Might Be) All You Need.bioRxiv : the preprint server for biology · 2024Article
- Bioelectric stimulation controls tissue shape and size.Nature communications · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Epithelial cells spontaneously form acini (also known as cysts or spheroids) with a single, fluid-filled central lumen when grown in 3D matrices. The size of the lumen is dependent on apical secretion of chloride ions, most notably by the CFTR channel, which has been suggested to establish pressure in the lumen due to water influx. To study the cellular biomechanics of acini morphogenesis and homeostasis, we used MDCK-2 cells. Using FRET-force biosensors for E-cadherin, we observed significant increases in the average tension per molecule for each protein in mature 3D acini as compared to 2D monolayers. Increases in CFTR activity resulted in increased E-cadherin forces, indicating that ionic gradients affect cellular tension. Direct measurements of pressure revealed that mature acini experience significant internal hydrostatic pressure (37 ± 10.9 Pa). Changes in CFTR activity resulted in pressure and/or volume changes, both of which affect E-cadherin tension. Increases in CFTR chloride secretion also induced YAP signaling and cellular proliferation. In order to recapitulate disruption of acinar homeostasis, we induced epithelial-to-mesenchymal transition (EMT). During the initial stages of EMT, there was a gradual decrease in E-cadherin force and lumen pressure that correlated with lumen infilling. Strikingly, increasing CFTR activity was sufficient to block EMT. Our results show that ion secretion is an important regulator of morphogenesis and homeostasis in epithelial acini. Furthermore, this work demonstrates that, for closed 3D cellular systems, ion gradients can generate osmotic pressure or volume changes, both of which result in increased cellular tension.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.