ArticlePlant biotechnology journal2020
Natural variation and CRISPR/Cas9-mediated mutation in GmPRR37 affect photoperiodic flowering and contribute to regional adaptation of soybean.
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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60 citing papers in PubMed, 136 citations in OpenAlex.
- Targeted allelic diversification of flowering genes via CRISPR-Cas enables the development of multiple early-flowering soybean lines.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Natural variation in GmRVE4d facilitates soybean latitudinal adaptation by regulating GmPRR5a-dependent flowering and maturity.Nature communications · 2026Article
- Advances in gene editing for legume improvement: technologies, progress, and prospects.Frontiers in genome editing · 2026Review
- Next-generation CRISPR systems and advanced delivery paradigms for precision crop improvement: a comparative case study in sugar beet (Frontiers in plant science · 2026Review
- Revolution and advances in gene editing and genomics technology for developing climate-resilient legume crops: developments and prospects.Plant molecular biology · 2025Review
- Using landscape genomics to infer genomic regions involved in environmental adaptation of soybean genebank accessions.BMC plant biology · 2025Article
- Transcriptional activation and repression in the plant circadian clock: Revisiting core oscillator feedback loops and output pathways.Plant communications · 2025Review
- CRISPR/Cas genome editing in soybean: challenges and new insights to overcome existing bottlenecks.Journal of advanced research · 2025Review
- The circadian clock module LgPRR7-LgFKF1 negatively regulates flowering time inHorticulture research · 2025Article
- Functional Verification of the Soybean Pseudo-Response FactorInternational journal of molecular sciences · 2025Article
- OsCKq1 Regulates Heading Date and Grain Weight in Rice in Response to Day Length.Rice (New York, N.Y.) · 2024Article
- Advancing PAM-less genome editing in soybean using CRISPR-SpRY.Horticulture research · 2024Article
- Flowering time regulator qFT13-3 involved in soybean adaptation to high latitudes.Plant biotechnology journal · 2024Article
- ZmELF6-ZmPRR37 module regulates maize flowering and salt response.Plant biotechnology journal · 2024Article
- Exploring genetic variability under extended photoperiod in lentil (Lens Culinaris Medik): vegetative and phenological differentiation according to genetic material's origins.Plant methods · 2024Article
- Modification of Fatty Acid Profile and Oil Contents Using Gene Editing in Oilseed Crops for a Changing Climate.GM crops & food · 2023Review
- Environmental F actors coordinate circadian clock function and rhythm to regulate plant development.Plant signaling & behavior · 2023Review
- Genome-wide association study reveals loci and candidate genes of flowering time in jute (Molecular breeding : new strategies in plant improvement · 2023Article
- Unlocking Nature's Clock: CRISPR Technology in Flowering Time Engineering.Plants (Basel, Switzerland) · 2023Review
- The Roles of theInternational journal of molecular sciences · 2023Article
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flowering time is a critical determinant of the geographic distribution and regional adaptability of soybean (Glycine max) and is strongly regulated by photoperiod and temperature. In this study, quantitative trait locus (QTL) mapping and subsequent candidate gene analysis revealed that GmPRR37, encoding a pseudo-response regulator protein, is responsible for the major QTL qFT12-2, which was identified from a population of 308 recombinant inbred lines (RILs) derived from a cross between a very late-flowering soybean cultivar, 'Zigongdongdou (ZGDD)', and an extremely early-flowering cultivar, 'Heihe27 (HH27)', in multiple environments. Comparative analysis of parental sequencing data confirmed that HH27 contains a non-sense mutation that causes the loss of the CCT domain in the GmPRR37 protein. CRISPR/Cas9-induced Gmprr37-ZGDD mutants in soybean exhibited early flowering under natural long-day (NLD) conditions. Overexpression of GmPRR37 significantly delayed the flowering of transgenic soybean plants compared with wild-type under long photoperiod conditions. In addition, both the knockout and overexpression of GmPRR37 in soybean showed no significant phenotypic alterations in flowering time under short-day (SD) conditions. Furthermore, GmPRR37 down-regulated the expression of the flowering-promoting FT homologues GmFT2a and GmFT5a, and up-regulated flowering-inhibiting FT homologue GmFT1a expression under long-day (LD) conditions. We analysed haplotypes of GmPRR37 among 180 cultivars collected across China and found natural Gmprr37 mutants flower earlier and enable soybean to be cultivated at higher latitudes. This study demonstrates that GmPRR37 controls soybean photoperiodic flowering and provides opportunities to breed optimized cultivars with adaptation to specific regions and farming systems.
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