ArticleMolecular human reproduction2020
Towards uterus tissue engineering: a comparative study of sheep uterus decellularisation.
Article in Molecular human reproduction, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Bioengineering trends in female reproduction: a systematic review.Human reproduction update · 2022Pooled it
- Ex vivo perfusion recellularization of acellular rat hindlimbs for vascular composite allotransplantation.Scientific reports · 2025Article
- Biomechanical and Compositional Changes in the Murine Uterus with Age.Annals of biomedical engineering · 2025Article
- Infertility treatment using polysaccharides-based hydrogels: new strategies in tissue engineering and regenerative medicine.Journal of nanobiotechnology · 2025Review
- Toward human uterus tissue engineering: Uterine decellularization in a non-human primate species.Acta obstetricia et gynecologica Scandinavica · 2025Article
- Standardizing decellularization protocols for optimized uterine tissue bioengineering.Regenerative therapy · 2025Review
- Application of decellularized tissue for soft-hard interregional regeneration.Frontiers in bioengineering and biotechnology · 2025Review
- Application of tergitol in liver decellularization and bioink preparation based on the obtained extracellular matrix.Frontiers in bioengineering and biotechnology · 2025Article
- Animal models of postpartum hemorrhage.Lab animal · 2024Review
- Comparative analysis of porcine-uterine decellularization for bioactive-molecule preservation and DNA removal.Frontiers in bioengineering and biotechnology · 2024Article
- Current status and challenges in uterine myometrial tissue engineering.Bioengineered · 2023Review
- New Solutions for Old Problems: How Reproductive Tissue Engineering Has Been Revolutionizing Reproductive Medicine.Annals of biomedical engineering · 2023Review
- Uterus transplantation: from research, through human trials and into the future.Human reproduction update · 2023Review
- A Decellularized Uterine Endometrial Scaffold Enhances Regeneration of the Endometrium in Rats.International journal of molecular sciences · 2023Article
- A Combination of Physical and Chemical Treatments Is More Effective in The Preparation of Acellular Uterine Scaffolds.Cell journal · 2023Article
- Intravital tumor decellularization as a new approach to cancer treatment.American journal of cancer research · 2023Article
- Whole-organ decellularization of the human uterus and in vivo application of the bio-scaffolds in animal models.Journal of assisted reproduction and genetics · 2022Article
- Future Challenges and Opportunities of Extracellular Matrix Hydrogels in Female Reproductive Medicine.International journal of molecular sciences · 2022Review
- Same-sex parenting according to the Italian Constitutional Court: the need for legislative solutions.Acta bio-medica : Atenei Parmensis · 2022Article
- Advancements in the Use of Hydrogels for Regenerative Medicine: Properties and Biomedical Applications.International journal of biomaterials · 2022Review
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Uterus tissue engineering may dismantle limitations in current uterus transplantation protocols. A uterine biomaterial populated with patient-derived cells could potentially serve as a graft to circumvent complicated surgery of live donors, immunosuppressive medication and rejection episodes. Repeated uterine bioengineering studies on rodents have shown promising results using decellularised scaffolds to restore fertility in a partially impaired uterus and now mandate experiments on larger and more human-like animal models. The aim of the presented studies was therefore to establish adequate protocols for scaffold generation and prepare for future in vivo sheep uterus bioengineering experiments. Three decellularisation protocols were developed using vascular perfusion through the uterine artery of whole sheep uteri obtained from slaughterhouse material. Decellularisation solutions used were based on 0.5% sodium dodecyl sulphate (Protocol 1) or 2% sodium deoxycholate (Protocol 2) or with a sequential perfusion of 2% sodium deoxycholate and 1% Triton X-100 (Protocol 3). The scaffolds were examined by histology, extracellular matrix quantification, evaluation of mechanical properties and the ability to support foetal sheep stem cells after recellularisation. We showed that a sheep uterus can successfully be decellularised while maintaining a high integrity of the extracellular components. Uteri perfused with sodium deoxycholate (Protocol 2) were the most favourable treatment in our study based on quantifications. However, all scaffolds supported stem cells for 2 weeks in vitro and showed no cytotoxicity signs. Cells continued to express markers for proliferation and maintained their undifferentiated phenotype. Hence, this study reports three valuable decellularisation protocols for future in vivo sheep uterus bioengineering experiments.
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