ArticleScientific reports2020
Quality control of multiplex antibody detection in samples from large-scale surveys: the example of malaria in Haiti.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Serological surveillance for malaria elimination in Southeast Asia: a scoping review.Malaria journal · 2026Article
- Article
- A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses.Journal of visualized experiments : JoVE · 2025Article
- shinyMBA: a novel R shiny application for quality control of the multiplex bead assay for serosurveillance studies.Scientific reports · 2024Article
- Antibody selection strategies and their impact in predicting clinical malaria based on multi-sera data.BioData mining · 2024Article
- Spatial Clustering and Risk Factors for Malaria Infections and Marker of Recent Exposure to Plasmodium falciparum from a Household Survey in Artibonite, Haiti.The American journal of tropical medicine and hygiene · 2023Article
- Analytical approaches for antimalarial antibody responses to confirm historical and recent malaria transmission: an example from the Philippines.The Lancet regional health. Western Pacific · 2023Article
- Acceptability, Feasibility, Drug Safety, and Effectiveness of a Pilot Mass Drug Administration with a Single Round of Sulfadoxine-Pyrimethamine Plus Primaquine and Indoor Residual Spraying in Communities with Malaria Transmission in Haiti, 2018.The American journal of tropical medicine and hygiene · 2023Article
- Non-falciparum malaria infection and IgG seroprevalence among children under 15 years in Nigeria, 2018.Nature communications · 2023Article
- Dynamics of IgG antibody response againstFrontiers in immunology · 2023Article
- Editorial: Current research on serological analyses of infectious diseases.Frontiers in medicine · 2023Article
- Article
- Factors Associated With Human IgG Antibody Response to Anopheles albimanus Salivary Gland Extract, Artibonite Department, Haiti, 2017.The Journal of infectious diseases · 2022Article
- Etramp5 as a useful serological marker in children to assess the immediate effects of mass drug campaigns for malaria.BMC infectious diseases · 2022Article
- Reactive focal drug administration associated with decreased malaria transmission in an elimination setting: Serological evidence from the cluster-randomized CoRE study.PLOS global public health · 2022Article
- Article
- Adaptation of ELISA detection of Plasmodium falciparum and Plasmodium vivax circumsporozoite proteins in mosquitoes to a multiplex bead-based immunoassay.Malaria journal · 2021Article
- Risk Factors for Malaria Infection and Seropositivity in the Elimination Area of Grand'Anse, Haiti: A Case-Control Study among Febrile Individuals Seeking Treatment at Public Health Facilities.The American journal of tropical medicine and hygiene · 2020Article
- Selection of Antibody Responses Associated WithFrontiers in immunology · 2020Article
Corrections and comments
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Authors and funding
19 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Measuring antimalarial antibodies can estimate transmission in a population. To compare outputs, standardized laboratory testing is required. Here we describe the in-country establishment and quality control (QC) of a multiplex bead assay (MBA) for three sero-surveys in Haiti. Total IgG data against 21 antigens were collected for 32,758 participants. Titration curves of hyperimmune sera were included on assay plates, assay signals underwent 5-parameter regression, and inspection of the median and interquartile range (IQR) for the y-inflection point was used to determine assay precision. The medians and IQRs were similar for Surveys 1 and 2 for most antigens, while the IQRs increased for some antigens in Survey 3. Levey-Jennings charts for selected antigens provided a pass/fail criterion for each assay plate and, of 387 assay plates, 13 (3.4%) were repeated. Individual samples failed if IgG binding to the generic glutathione-S-transferase protein was observed, with 659 (2.0%) samples failing. An additional 455 (1.4%) observations failed due to low bead numbers (<20/analyte). The final dataset included 609,438 anti-malaria IgG data points from 32,099 participants; 96.6% of all potential data points if no QC failures had occurred. The MBA can be deployed with high-throughput data collection and low inter-plate variability while ensuring data quality.
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