ArticleNature communications2020
Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics.
Erratum issuedOpen access · goldAbstract read
In one paragraph
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 93 papers.
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93citing papers in PubMed
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Who cites it
93 citing papers in PubMed, 141 citations in OpenAlex.
- Effect of glucagon like peptide-1 receptor agonist exenatide, used as an intracranial pressure lowering agent, on cognition in Idiopathic Intracranial Hypertension.Eye (London, England) · 2024Trial
- The effect of GLP-1RA exenatide on idiopathic intracranial hypertension: a randomized clinical trial.Brain : a journal of neurology · 2023Trial
- GLP-1 Receptor Agonist and GIP/GLP-1 Receptor Dual Agonist Therapeutics at the Intersection of Alcohol Use Disorder, Obesity, and Cardiometabolic Dysfunction.Biological psychiatry global open science · 2026Review
- Islet ultrastructure: past achievements and future directions.Diabetologia · 2026Review
- Primary cilia regulate GLP-1 signaling in pancreatic β cells.Molecular metabolism · 2026Article
- Brainstem GLP-1 neurons modulate physiological satiation and drive sustained weight loss in obese mice.Molecular metabolism · 2026Article
- Acute Contractile Effects of Glucagon-like-Peptide-1 Receptor Agonists in the Human Heart.Pharmaceutics · 2026Review
- Lysine Targeting Group-Transfer Chimeras for Proximity Induction.Angewandte Chemie (International ed. in English) · 2026Article
- Survodutide acts through circumventricular organs in the brain and activates neuronal regions associated with appetite regulation.Molecular metabolism · 2026Article
- In vivo functional profiling and structural characterization of the humanScience advances · 2026Article
- Papilledema is not the point: intracranial pressure dysregulation as a common pathway in migraine and idiopathic intracranial hypertension.Brain communications · 2026Article
- Individual paraventricular hypothalamic GLP-1R neurons track ingestion across energy states.iScience · 2025Article
- Downstream interaction by glucagon-like peptide-1 and glucose-dependent insulinotropic polypeptide agonism is required for synergistic effects on body weight.Molecular metabolism · 2025Article
- Localized GLP1 receptor pre-internalization directs pancreatic alpha cell to beta cell communication.Cell metabolism · 2025Article
- Lighting up targets of dual agonist therapies for diabetes and obesity.Nature metabolism · 2025Article
- Fluorescent GLP1R/GIPR dual agonist probes reveal cell targets in the pancreas and brain.Nature metabolism · 2025Article
- Article
- Current understanding and controversy on brain access of GLP-1 and GLP-1 receptor agonists.Journal of translational internal medicine · 2025Article
- Glucagon-Like Peptide-1 and Hypothalamic Regulation of Satiation: Cognitive and Neural Insights from Human and Animal Studies.Diabetes & metabolism journal · 2025Review
- Antidiabetic GLP-1 Receptor Agonists Have Neuroprotective Properties in Experimental Animal Models of Alzheimer's Disease.Pharmaceuticals (Basel, Switzerland) · 2025Review
33 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
26 authors at 11 institutions in 4 countries.
Julia AstInstitute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.ORCID http://orcid.org/0000-0002-0039-4762
Anastasia ArvanitiInstitute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.
Nicholas H F FineInstitute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.ORCID http://orcid.org/0000-0003-2343-8534
Daniela NasteskaInstitute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.ORCID http://orcid.org/0000-0002-8996-5102
Fiona B AshfordInstitute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.
Zania StamatakiCentre for Liver Research, College of Medical and Dental Sciences, Institute for Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.ORCID http://orcid.org/0000-0003-3823-4497
Zsombor KoszegiInstitute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.
Andrea BaconGenome Editing Facility, Technology Hub, University of Birmingham, Birmingham, UK.
Ben J JonesDivision of Diabetes, Endocrinology and Metabolism, Section of Investigative Medicine, Imperial College London, London, UK.
Maria A LuceyDivision of Diabetes, Endocrinology and Metabolism, Section of Investigative Medicine, Imperial College London, London, UK.
Shugo SasakiDiabetes Research Group, BC Children's Hospital Research Institute, Vancouver, BC, Canada; Department of Surgery, University of British Columbia, Vancouver, BC, Canada.ORCID http://orcid.org/0000-0002-3696-7809
Daniel I BrierleyCentre for Cardiovascular and Metabolic Neuroscience, Department of Neuroscience, Physiology & Pharmacology, University College London, London, UK.ORCID http://orcid.org/0000-0002-4360-2648
Benoit HastoyOxford Centre for Diabetes, Endocrinology & Metabolism, University of Oxford, Oxford, UK.ORCID http://orcid.org/0000-0003-1244-7857
Alejandra TomasDivision of Diabetes, Endocrinology and Metabolism, Section of Cell Biology and Functional Genomics, Imperial College London, London, UK.
Giuseppe D'AgostinoFaculty of Biology, Medicine and Health, University of Manchester, Oxford Road, Manchester, UK.ORCID http://orcid.org/0000-0002-3502-4251
Frank ReimannWellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0001-9399-6377
Francis C LynnDiabetes Research Group, BC Children's Hospital Research Institute, Vancouver, BC, Canada; Department of Surgery, University of British Columbia, Vancouver, BC, Canada.ORCID http://orcid.org/0000-0001-9318-1063
Christopher A ReissausDepartment of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA.
Amelia K LinnemannDepartment of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA.ORCID http://orcid.org/0000-0001-7356-4876
Elisa D'EsteOptical Microscopy Facility, Max Planck Institute for Medical Research, Heidelberg, Germany.
Davide CalebiroInstitute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.ORCID http://orcid.org/0000-0002-3811-1553
Stefan TrappCentre for Cardiovascular and Metabolic Neuroscience, Department of Neuroscience, Physiology & Pharmacology, University College London, London, UK.ORCID http://orcid.org/0000-0003-0665-4948
Kai JohnssonDepartment of Chemical Biology, Max Planck Institute for Medical Research, Heidelberg, Germany.ORCID http://orcid.org/0000-0002-8002-1981
Tom PodewinDepartment of Chemical Biology, Max Planck Institute for Medical Research, Heidelberg, Germany. tom.podewin@mpimf-heidelberg.mpg.de.ORCID http://orcid.org/0000-0002-1632-5104
Johannes BroichhagenDepartment of Chemical Biology, Max Planck Institute for Medical Research, Heidelberg, Germany. johannes.broichhagen@mr.mpg.de.ORCID http://orcid.org/0000-0003-3084-6595
David J HodsonInstitute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK. d.hodson@bham.ac.uk.ORCID http://orcid.org/0000-0002-8641-8568
University of Birmingham · GBMax Planck Institute for Medical Research · DEImperial College London · GBIndiana University School of MedicineUniversity College London · GBUniversity of British Columbia · CADiabetes UK · GBGenomics (United Kingdom) · GBUniversity of Cambridge · GBUniversity of Manchester · GBUniversity of Oxford · GB
Funding
Translation CoreP30DK097512 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI Carmella Evans-Molina · 2015 to 2026
$17.4MRenewal of the Human Islet Research Enhancement Center (HIREC) for the Type-1-Diabetes-Focused Human Islet Research Network (HIRN).U24DK104162 · NIDDK · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI John S. Kaddis, Joyce Carol Niland · 2019 to 2026
$12.3MHuman Islet Research Network (HIRN) Coordinating CenterU01DK104162 · NIDDK · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI NILAND, JOYCE CAROL · 2014 to 2018
$6.1MRESEARCH TRAINING PROGRAM IN DIABETES AND OBESITYT32DK064466 · NIDDK · INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS · PI Carmella Evans-Molina, RONALD C WEK · 2003 to 2026
$5.2MNeuroendocrine Integration of Satiety and Food RewardR01DK095757 · NIDDK · FLORIDA STATE UNIVERSITY · PI WILLIAMS, DIANA L · 2013 to 2022
$3.3MMechanisms of IL-6 Signaling in Beta-cell Stress ResponseR03DK115990 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI LINNEMANN, AMELIA K · 2018 to 2019
$236kMedical Research Council MC_UU_00014/3Medical Research Council MC_UU_00014/5Medical Research Council MC_UU_12012/3Medical Research Council MR/K023667/1Medical Research Council MR/N00275X/1Medical Research Council MR/N02589X/1Medical Research Council MR/P009824/1Medical Research Council MR/P009824/2Medical Research Council MR/R010676/1Medical Research Council MR/S025618/1NIDDK NIH HHS P30 DK097512NIDDK NIH HHS R01 DK095757NIDDK NIH HHS R03 DK115990NIDDK NIH HHS T32 DK064466NIDDK NIH HHS U01 DK104162NIDDK NIH HHS U24 DK104162Wellcome Trust
Abstract
The glucagon-like peptide-1 receptor (GLP1R) is a class B G protein-coupled receptor (GPCR) involved in metabolism. Presently, its visualization is limited to genetic manipulation, antibody detection or the use of probes that stimulate receptor activation. Herein, we present LUXendin645, a far-red fluorescent GLP1R antagonistic peptide label. LUXendin645 produces intense and specific membrane labeling throughout live and fixed tissue. GLP1R signaling can additionally be evoked when the receptor is allosterically modulated in the presence of LUXendin645. Using LUXendin645 and LUXendin651, we describe islet, brain and hESC-derived β-like cell GLP1R expression patterns, reveal higher-order GLP1R organization including membrane nanodomains, and track single receptor subpopulations. We furthermore show that the LUXendin backbone can be optimized for intravital two-photon imaging by installing a red fluorophore. Thus, our super-resolution compatible labeling probes allow visualization of endogenous GLP1R, and provide insight into class B GPCR distribution and dynamics both in vitro and in vivo.
Indexed as
Fluorescent DyesAmino Acid SequenceAnimalsBrainCell LineGlucagon-Like Peptide-1 ReceptorHEK293 CellsHuman Embryonic Stem CellsHumansIslets of LangerhansMiceMice, KnockoutMicroscopy, Fluorescence, MultiphotonModels, MolecularMolecular StructurePeptide Fragmentsexendin (9-39)Fluorescent DyesGLP1R protein, humanGlp1r protein, mouseGlucagon-Like Peptide-1 ReceptorPeptide Fragments
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.