ArticleThe Journal of biological chemistry2020
Improved workflow for mass spectrometry-based metabolomics analysis of the heart.
Article in The Journal of biological chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Metabolomics and metabolites in cancer diagnosis and treatment.Molecular biomedicine · 2025Pooled it
- Targeted metabolomics of postmortem human cardiac tissue using the Biocrates MxP Quant 500 kit.PloS one · 2026Article
- Anesthesia, perimortem conditions, and brain harvest method govern the measured metabolome: analysis of substandard samples generates flawed metabolite levels.Oxford open neuroscience · 2026Article
- Brain Glycogen-Its Metabolic Role in Neuronal Health and Neurological Disorders-An Extensive Narrative Review.Metabolites · 2025Review
- Jellyfish stings-induced cardiac failure was ameliorated through AAG-mediated glycogen-driven ATP production.Exploration (Beijing, China) · 2025Article
- TgLaforin, a glucan phosphatase, reveals the dynamic role of storage polysaccharides inbioRxiv : the preprint server for biology · 2024Article
- The multifaceted roles of the brain glycogen.Journal of neurochemistry · 2024Review
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- Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosis.Nature communications · 2023Article
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- Article
- Metabolomics meets systems immunology.EMBO reports · 2023Review
- Cervical spinal cord injury leads to injury and altered metabolism in the lungs.Brain communications · 2023Article
- In situ mass spectrometry imaging reveals heterogeneous glycogen stores in human normal and cancerous tissues.EMBO molecular medicine · 2022Article
- Tissue-Specific Downregulation of Fatty Acid Synthase Suppresses Intestinal Adenoma Formation via Coordinated Reprograming of Transcriptome and Metabolism in the Mouse Model of Apc-Driven Colorectal Cancer.International journal of molecular sciences · 2022Article
- Optimization of metabolomic data processing using NOREVA.Nature protocols · 2022Review
- Lactate supports a metabolic-epigenetic link in macrophage polarization.Science advances · 2021Article
- APOΕ4 lowers energy expenditure in females and impairs glucose oxidation by increasing flux through aerobic glycolysis.Molecular neurodegeneration · 2021Article
- Brain glycogen serves as a critical glucosamine cache required for protein glycosylation.Cell metabolism · 2021Article
- Targeting Adrenergic Receptors in Metabolic Therapies for Heart Failure.International journal of molecular sciences · 2021Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
MS-based metabolomics methods are powerful techniques to map the complex and interconnected metabolic pathways of the heart; however, normalization of metabolite abundance to sample input in heart tissues remains a technical challenge. Herein, we describe an improved GC-MS-based metabolomics workflow that uses insoluble protein-derived glutamate for the normalization of metabolites within each sample and includes normalization to protein-derived amino acids to reduce biological variation and detect small metabolic changes. Moreover, glycogen is measured within the metabolomics workflow. We applied this workflow to study heart metabolism by first comparing two different methods of heart removal: the Langendorff heart method (reverse aortic perfusion) and
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