ArticleBMC microbiology2020
Comparative genomics of multidrug-resistant Enterococcus spp. isolated from wastewater treatment plants.
Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 48 citations in OpenAlex.
- Antimicrobial Resistance Gene Profiles inPathogens (Basel, Switzerland) · 2026Article
- Clade-specific evolution and genome plasticity underlying antimicrobial resistance in Enterococcus faecium.Archives of microbiology · 2026Review
- Molecular Epidemiology, Phenotypic and Genomic Characterization of Multidrug-Resistant Enterococcus Faecium Isolated from Bovine Mastitis in Ningxia, China (2019-2024).Microorganisms · 2026Article
- Advancements in CRISPR-Cas Systems for Genome Editing towards Eradication of Human Microbial Pathogens.Molecular biotechnology · 2026Review
- Article
- Evaluation of the antimicrobial susceptibility and resistance gene mapping ofOpen veterinary journal · 2026Article
- Metagenomic profiling of pathogens and antibiotic resistome in influent of six municipal wastewater treatment plants: a descriptive analysis of plant-specific microbial hazards.Frontiers in microbiology · 2026Article
- Wastewater treatment plant effluent alters particle-associated bacterial assemblages in an intermittent stream.Frontiers in microbiology · 2026Article
- The surveillance of antimicrobial resistance in wastewater from a one health perspective: A global scoping and temporal review (2014-2024).One health (Amsterdam, Netherlands) · 2025Article
- Development and application of a rapid dual RPA-LFS assay for simultaneous detection of Enterococcus faecalis and Enterococcus faecium.World journal of microbiology & biotechnology · 2025Article
- Comparative Analysis of Two Consecutive Genome Sequencing Results ofArchives of Razi Institute · 2025Article
- The spread of antimicrobial resistance in the aquatic environment from faecal pollution: a scoping review of a multifaceted issue.Environmental monitoring and assessment · 2025Article
- Bacterial Communities and Resistance and Virulence Genes in Hospital and Community Wastewater: Metagenomic Analysis.International journal of molecular sciences · 2025Article
- Article
- CRISPR-Cas systems in enterococci.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Review
- Comparative evaluation of commercial DNA isolation approaches for nanopore-only bacterial genome assembly and plasmid recovery.Scientific reports · 2024Article
- Article
- Multidrug-ResistantMicroorganisms · 2024Article
- Sub-MIC antibiotics increased the fitness cost of CRISPR-Cas inFrontiers in microbiology · 2024Article
- Dissemination of Enterococcal Genetic Lineages: A One Health Perspective.Antibiotics (Basel, Switzerland) · 2023Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundWastewater treatment plants (WWTPs) are considered hotspots for the environmental dissemination of antimicrobial resistance (AMR) determinants. Vancomycin-Resistant Enterococcus (VRE) are candidates for gauging the degree of AMR bacteria in wastewater. Enterococcus faecalis and Enterococcus faecium are recognized indicators of fecal contamination in water. Comparative genomics of enterococci isolated from conventional activated sludge (CAS) and biological aerated filter (BAF) WWTPs was conducted.
resultsVRE isolates, including E. faecalis (n = 24), E. faecium (n = 11), E. casseliflavus (n = 2) and E. gallinarum (n = 2) were selected for sequencing based on WWTP source, species and AMR phenotype. The pangenomes of E. faecium and E. faecalis were both open. The genomic fraction related to the mobilome was positively correlated with genome size in E. faecium (p < 0.001) and E. faecalis (p < 0.001) and with the number of AMR genes in E. faecium (p = 0.005). Genes conferring vancomycin resistance, including vanA and vanM (E. faecium), vanG (E. faecalis), and vanC (E. casseliflavus/E. gallinarum), were detected in 20 genomes. The most prominent functional AMR genes were efflux pumps and transporters. A minimum of 16, 6, 5 and 3 virulence genes were detected in E. faecium, E. faecalis, E. casseliflavus and E. gallinarum, respectively. Virulence genes were more common in E. faecalis and E. faecium, than E. casseliflavus and E. gallinarum. A number of mobile genetic elements were shared among species. Functional CRISPR/Cas arrays were detected in 13 E. faecalis genomes, with all but one also containing a prophage. The lack of a functional CRISPR/Cas arrays was associated with multi-drug resistance in E. faecium. Phylogenetic analysis demonstrated differential clustering of isolates based on original source but not WWTP. Genes related to phage and CRISPR/Cas arrays could potentially serve as environmental biomarkers.
conclusionsThere was no discernible difference between enterococcal genomes from the CAS and BAF WWTPs. E. faecalis and E. faecium have smaller genomes and harbor more virulence, AMR, and mobile genetic elements than other Enterococcus spp.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.