ArticleInternational journal of molecular sciences2020
Platelet Adhesion and Thrombus Formation in Microchannels: The Effect of Assay-Dependent Variables.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- The Role of Platelet-Derived Microparticles in Platelet Adhesion and Aggregation to Immobilized Extracellular Matrix Proteins Under Flow.Cellular and molecular bioengineering · 2026Article
- High glucose enhances inflammation-driven platelet adhesion to endothelial cells in vitro.Scientific reports · 2025Article
- Comparative study of flow rate- and material-dependent human plasma protein adsorption on oxygenator membranes and heat exchanger materials.Frontiers in cardiovascular medicine · 2025Article
- Low-Grade Inflammation in Long COVID Syndrome Sustains a Persistent Platelet Activation Associated With Lung Impairment.JACC. Basic to translational science · 2025Article
- Complex Pattern of Platelet Activation/Reactivity After SARS-CoV-2 Infection.International journal of molecular sciences · 2024Review
- Xylitol is prothrombotic and associated with cardiovascular risk.European heart journal · 2024Article
- Influence of Hematocrit Level and Integrin αCellular and molecular bioengineering · 2024Article
- Single molecule studies of dynamic platelet interactions with endothelial cells.Frontiers in bioengineering and biotechnology · 2024Article
- The artificial sweetener erythritol and cardiovascular event risk.Nature medicine · 2023Article
- Specific inhibition of the transporter MRP4/ABCC4 affects multiple signaling pathways and thrombus formation in human platelets.Haematologica · 2022Article
- Platelet activation and modulation in thrombosis with thrombocytopenia syndrome associated with ChAdO×1 nCov-19 vaccine.Haematologica · 2021Article
- Ultrastructural Study of Platelet Behavior and Interrelationship in Sprouting and Intussusceptive Angiogenesis during Arterial Intimal Thickening Formation.International journal of molecular sciences · 2021Article
- Vascular protective effect of aspirin and rivaroxaban upon endothelial denudation of the mouse carotid artery.Scientific reports · 2020Article
- Molecular Research on Platelet Activity in Health and Disease.International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Microfluidic flow chambers (MFCs) allow the study of platelet adhesion and thrombus formation under flow, which may be influenced by several variables. We developed a new MFC, with which we tested the effects of different variables on the results of platelet deposition and thrombus formation on a collagen-coated surface.
methodsWhole blood was perfused in the MFC over collagen Type I for 4 min at different wall shear rates (WSR) and different concentrations of collagen-coating solutions, keeping blood samples at room temperature or 37 °C before starting the experiments. In addition, we tested the effects of the antiplatelet agent acetylsalicylic acid (ASA) (antagonist of cyclooxygenase-1, 100 µM) and cangrelor (antagonist of P2Y
resultsPlatelet deposition on collagen (I) was not affected by the storage temperature of the blood before perfusion (room temperature vs. 37 °C); (II) was dependent on a shear rate in the range between 300/s and 1700/s; and (III) was influenced by the collagen concentration used to coat the microchannels up to a value of 10 µg/mL. ASA and cangrelor did not cause statistically significant inhibition of platelet accumulation, except for ASA at low collagen concentrations.
conclusionsPlatelet deposition on collagen-coated surfaces is a shear-dependent process, not influenced by the collagen concentration beyond a value of 10 µg/mL. However, the inhibitory effect of antiplatelet drugs is better observed using low concentrations of collagen.
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