Evidence map›Paper›PMID 31979077›Full record

ArticleInternational journal of molecular sciences2020

Validation of a New Multicistronic Plasmid for the Efficient and Stable Expression of Transgenes in Microalgae.

Ana Molina-Márquez, Marta Vila, Rocío Rengel, Emilio Fernández, Federico García-Maroto, Javier Vigara, Rosa León

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Ana Molina-MárquezLaboratory of Biochemistry. Faculty of Experimental Sciences. Marine International Campus of Excellence and RENSMA. University of Huelva, 21071 Huelva, Spain.
Marta VilaLaboratory of Biochemistry. Faculty of Experimental Sciences. Marine International Campus of Excellence and RENSMA. University of Huelva, 21071 Huelva, Spain.
Rocío RengelLaboratory of Biochemistry. Faculty of Experimental Sciences. Marine International Campus of Excellence and RENSMA. University of Huelva, 21071 Huelva, Spain.
Emilio FernándezDepartment of Biochemistry and Molecular Biology. University of Córdoba, 14071 Córdoba, Spain.
Federico García-MarotoLaboratory of Biotechnology of Natural Products, Agro-feed International Excellence campus, University of Almería, 04071 Almería, Spain.
Javier VigaraLaboratory of Biochemistry. Faculty of Experimental Sciences. Marine International Campus of Excellence and RENSMA. University of Huelva, 21071 Huelva, Spain.
Rosa LeónLaboratory of Biochemistry. Faculty of Experimental Sciences. Marine International Campus of Excellence and RENSMA. University of Huelva, 21071 Huelva, Spain.
Universidad de Huelva · ESUniversity of Almería · ESUniversity of Córdoba · ES

Funding

Interreg 0055 ALGARED PLUS 5Ministerio de Economía, Industria y Competitividad, Gobierno de España AGL2016-74866-C3-2-R
6 · The paper itself

Abstract

Low stability of transgenes and high variability of their expression levels among the obtained transformants are still pending challenges in the nuclear genetic transformation of microalgae. We have generated a new multicistronic microalgal expression plasmid, called Phyco69, to make easier the large phenotypic screening usually necessary for the selection of high-expression stable clones. This plasmid contains a polylinker region (PLK) where any gene of interest (

Indexed as

Anti-Bacterial AgentsGenetic MarkersKanamycin KinaseMicroalgaeParomomycinPlasmidsPromoter Regions, GeneticStreptomycesTransformation, GeneticTransgenesAnti-Bacterial AgentsGenetic MarkersKanamycin KinaseParomomycin2Amicroalgae transformationmulticistronic transcript.paromomycin

Identifiers

PMID31979077
PMCPMC7037629
OpenAlexW3002456264

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.