ArticleCell2020
Snake Venom Gland Organoids.
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Article in Cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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65citing papers in PubMed
14.2field-weighted citation impact, top 1% of its field
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65 citing papers in PubMed, 116 citations in OpenAlex.
- Animal organoids as models for integrated One Health research.One health (Amsterdam, Netherlands) · 2026Review
- Targeting snake venom metalloproteinases: structural diversity, molecular pathogenesis and therapeutic interventions.Molecular biology reports · 2026Review
- Lessons on multicellular two- (2D) and three-dimensional (3D) culture in parasitology: Insights, challenges and future directions.International journal for parasitology · 2026Review
- Organoids: From Bench to Bedside Applications.MedComm · 2026Review
- Parabiosis, Assembloids, Organoids (PAO).Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Development and characterization of chicken lung organoids for in vitro modeling of avian influenza virus-host cell interaction.Scientific reports · 2026Article
- Transcriptomic Insights Into the Evolution of Snake Venom: Mechanisms, Diversity, and Adaptation.Scientifica · 2026Review
- Article
- Therapeutic Promise and Biotechnological Prospects ofInternational journal of molecular sciences · 2025Review
- Article
- The gene regulatory mechanisms shaping the heterogeneity of venom production in the Cape coral snake.Genome biology · 2025Article
- Long-term culture of chicken tracheal organoids for the purpose of avian influenza virus research.Virology journal · 2025Article
- Integrin-activatingProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Advancing stem cell technologies for conservation of wildlife biodiversity.Development (Cambridge, England) · 2024Review
- Article
- Primary Cell Culture as a Model System for Evolutionary Molecular Physiology.International journal of molecular sciences · 2024Review
- The impact of induced pluripotent stem cells in animal conservation.Veterinary research communications · 2024Review
- Bioengineered Organoids Offer New Possibilities for Liver Cancer Studies: A Review of Key Milestones and Challenges.Bioengineering (Basel, Switzerland) · 2024Review
- Establishment and characterization of turtle liver organoids provides a potential model to decode their unique adaptations.Communications biology · 2024Article
- Distinct regulatory networks control toxin gene expression in elapid and viperid snakes.BMC genomics · 2024Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
31 authors at 11 institutions in 2 countries.
Yorick PostHubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands; Oncode Institute, Hubrecht Institute, 3584 CT Utrecht, the Netherlands.
Jens PuschhofHubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands; Oncode Institute, Hubrecht Institute, 3584 CT Utrecht, the Netherlands.
Joep BeumerHubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands; Oncode Institute, Hubrecht Institute, 3584 CT Utrecht, the Netherlands.
Harald M KerkkampNaturalis Biodiversity Center, 2333 CR Leiden, the Netherlands; Institute of Biology Leiden, Department of Animal Science and Health, 2333 BE Leiden, the Netherlands.
Merijn A G de BakkerInstitute of Biology Leiden, Department of Animal Science and Health, 2333 BE Leiden, the Netherlands.
Julien SlagboomDivision of BioAnalytical Chemistry, Department of Chemistry and Pharmaceutical Sciences, Vrije Universiteit Amsterdam, 1081 LA Amsterdam, the Netherlands.
Buys de BarbansonHubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands; Oncode Institute, Hubrecht Institute, 3584 CT Utrecht, the Netherlands.
Nienke R WeversMimetas BV, Organ-on-a-Chip Company, 2333 CH Leiden, the Netherlands; Department of Cell and Chemical Biology, Leiden University Medical Centre, Einthovenweg 20, 2333 ZC Leiden, the Netherlands.
Xandor M SpijkersMimetas BV, Organ-on-a-Chip Company, 2333 CH Leiden, the Netherlands; Department of Translational Neuroscience, Utrecht University Medical Center, 3584 CG Utrecht, the Netherlands.
Thomas OlivierMimetas BV, Organ-on-a-Chip Company, 2333 CH Leiden, the Netherlands.
Taline D KazandjianCentre for Snakebite Research & Interventions, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK.
Stuart AinsworthCentre for Snakebite Research & Interventions, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK.
Carmen Lopez IglesiasThe Maastricht Multimodal Molecular Imaging Institute, Maastricht University, 6229 ER Maastricht, the Netherlands.
Willine J van de WeteringHubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, 6229 ER Maastricht, the Netherlands.
Maria C HeinzOncode Institute, Hubrecht Institute, 3584 CT Utrecht, the Netherlands; Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, 3584 CX Utrecht, the Netherlands.
Ravian L van IneveldOncode Institute, Hubrecht Institute, 3584 CT Utrecht, the Netherlands; The Princess Maxima Center for Pediatric Oncology, 3584 CS Utrecht, the Netherlands.
Regina G D M van KleefNeurotoxicology Research Group, Division of Toxicology, Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3584 CL Utrecht, the Netherlands.
Harry BegthelHubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands; Oncode Institute, Hubrecht Institute, 3584 CT Utrecht, the Netherlands.
Jeroen KorvingHubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands; Oncode Institute, Hubrecht Institute, 3584 CT Utrecht, the Netherlands.
Yotam E Bar-EphraimHubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands; Oncode Institute, Hubrecht Institute, 3584 CT Utrecht, the Netherlands.
Walter GetreuerSerpo, 2288 ED Rijswijk, the Netherlands.
Anne C RiosOncode Institute, Hubrecht Institute, 3584 CT Utrecht, the Netherlands; The Princess Maxima Center for Pediatric Oncology, 3584 CS Utrecht, the Netherlands.
Remco H S WesterinkNeurotoxicology Research Group, Division of Toxicology, Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3584 CL Utrecht, the Netherlands.
Hugo J G SnippertOncode Institute, Hubrecht Institute, 3584 CT Utrecht, the Netherlands; Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, 3584 CX Utrecht, the Netherlands.
Alexander van OudenaardenHubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands; Oncode Institute, Hubrecht Institute, 3584 CT Utrecht, the Netherlands.
Peter J PetersThe Maastricht Multimodal Molecular Imaging Institute, Maastricht University, 6229 ER Maastricht, the Netherlands.
Freek J VonkNaturalis Biodiversity Center, 2333 CR Leiden, the Netherlands.
Jeroen KoolDivision of BioAnalytical Chemistry, Department of Chemistry and Pharmaceutical Sciences, Vrije Universiteit Amsterdam, 1081 LA Amsterdam, the Netherlands; Division of BioAnalytical Chemistry, Department of Chemistry and Pharmaceutical Sciences, Amsterdam Institute for Molecules Medicines and Systems, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, the Netherlands.
Michael K RichardsonInstitute of Biology Leiden, Department of Animal Science and Health, 2333 BE Leiden, the Netherlands.
Nicholas R CasewellCentre for Snakebite Research & Interventions, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK.
Hans CleversHubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands; Oncode Institute, Hubrecht Institute, 3584 CT Utrecht, the Netherlands; The Princess Maxima Center for Pediatric Oncology, 3584 CS Utrecht, the Netherlands. Electronic address: h.clevers@hubrecht.eu.
Oncode Institute · NLRoyal Netherlands Academy of Arts and Sciences · NLLiverpool School of Tropical Medicine · GBUtrecht University · NLLeiden University · NLMaastricht University · NLMimetas (Netherlands) · NLNaturalis Biodiversity Center · NLPrincess Máxima Center · NLVrije Universiteit Amsterdam · NLEuropean Patent Organisation · NL
Funding
Wellcome Trust 200517/Z/16/Z
Abstract
Wnt dependency and Lgr5 expression define multiple mammalian epithelial stem cell types. Under defined growth factor conditions, such adult stem cells (ASCs) grow as 3D organoids that recapitulate essential features of the pertinent epithelium. Here, we establish long-term expanding venom gland organoids from several snake species. The newly assembled transcriptome of the Cape coral snake reveals that organoids express high levels of toxin transcripts. Single-cell RNA sequencing of both organoids and primary tissue identifies distinct venom-expressing cell types as well as proliferative cells expressing homologs of known mammalian stem cell markers. A hard-wired regional heterogeneity in the expression of individual venom components is maintained in organoid cultures. Harvested venom peptides reflect crude venom composition and display biological activity. This study extends organoid technology to reptilian tissues and describes an experimentally tractable model system representing the snake venom gland.
Indexed as
Adult Stem CellsAnimalsCell Culture TechniquesCoral SnakesGene Expression ProfilingOrganoidsSalivary GlandsSnakesSnake VenomsStem CellsToxins, BiologicalTranscriptomeSnake VenomsToxins, Biologicalheterogeneityknock-in reporterLgr5organoidsingle cell RNA sequencingsnakestem cellstranscriptomicsvenom gland
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.