ArticlePloS one2020
Effect of Lactobacillus acidophilus D2/CSL (CECT 4529) supplementation in drinking water on chicken crop and caeca microbiome.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Probiotics in Poultry: A Comprehensive Review of Mechanisms, Applications, and Future Directions.Veterinary sciences · 2026Review
- Reducing Antimicrobial Resistance in Poultry Carcasses Extends Beyond Farm-Level Interventions.Foods (Basel, Switzerland) · 2026Article
- Evaluating the efficacy of compound probiotics as an antibiotic alternative: A comprehensive analysis of growth performance and intestinal health via feed and water routes.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- DietaryFoods (Basel, Switzerland) · 2024Article
- Microbial contamination pathways in a poultry abattoir provided clues on the distribution and persistence ofApplied and environmental microbiology · 2024Article
- Effects of Eimeria acervulina infection on the luminal and mucosal microbiota of the cecum and ileum in broiler chickens.Scientific reports · 2024Article
- Effects of Designed Herbal Formula on Growth Performance, Blood Indices, Organ Traits, and Cecum Microbiology in Broilers.Veterinary sciences · 2024Article
- Effects of Dietary Supplementation ofAnimals : an open access journal from MDPI · 2022Article
- Efficacy of Microencapsulated Probiotic as Adjunct Therapy on Resolution of Diarrhea, Copper-Zinc Homeostasis, Immunoglobulins, and Inflammatory Markers in Serum of Spontaneous Rotavirus-Infected Diarrhoetic Calves.Probiotics and antimicrobial proteins · 2022Article
- Comparing western (Megascops kennicottii) and whiskered (M. trichopsis) screech-owl microbiomes in southern Arizona using a novel 16S rRNA sequencing method.Animal microbiome · 2022Article
- Influence of the Gut Microbiome on Feed Intake of Farm Animals.Microorganisms · 2022Review
- Article
- Article
- Cigarette smoking status alters dysbiotic gut microbes in hypertensive patients.Journal of clinical hypertension (Greenwich, Conn.) · 2021Article
- Comparison between 16S rRNA and shotgun sequencing data for the taxonomic characterization of the gut microbiota.Scientific reports · 2021Article
- Pretreatment with probiotics ameliorate gut health and necrotic enteritis in broiler chickens, a substitute to antibiotics.AMB Express · 2020Review
- Effect of Heat-Inactivated Compound Probiotics on Growth Performance, Plasma Biochemical Indices, and Cecal Microbiome in Yellow-Feathered Broilers.Frontiers in microbiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we gained insights into the effects of the supplementation with Lactobacillus acidophilus D2/CSL (CECT 4529) in the chicken drinking water on crop and caeca microbiomes. The probiotic was supplemented at the concentrations of 0.2 g Lactobacillus acidophilus/day/bird and 0.02 g Lactobacillus acidophilus/day/bird and its effect on the crop and caeca microbiomes was assessed at 14 and 35 days of rearing. The results showed that mean relative abundance of Lactobacillus acidophilus in the caeca did not show significative differences in the treated and control birds, although Lactobacillus acidophilus as well as Faecalibacterium prausnitzii, Lactobacillus crispatus and Lactobacillus reuteri significantly increased over time. Moreover, the treatment with the high dose of probiotic significantly increased the abundance of Clostridium asparagiforme, Clostridium hathewayi and Clostridium saccharolyticum producing butyrate and other organic acids supporting the chicken health. Finally, at 35 days, the Cell division protein FtsH (EC 3.4.24.-) and the Site-specific recombinase genes were significantly increased in the caeca of birds treated with the high dose of probiotic in comparison to the control group. The results of this study showed that Lactobacillus acidophilus D2/CSL (CECT 4529) supplementation in the drinking water at the concentrations of 0.2 and 0.02 g Lactobacillus acidophilus/day/bird improved beneficial microbes and functional genes in broiler crops and caeca. Nevertheless, the main site of action of the probiotic is the crop, at least in the early stage of the chicken life. Indeed, at 14 days Lactobacillus acidophilus was significantly higher in the crops of chickens treated with the high dose of LA in comparison to the control (14.094 vs 1.741%, p = 0.036).
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