ArticlePloS one2020
Switchable resolution in soft x-ray tomography of single cells.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Interior soft x-ray tomography with sparse global sampling.Physica scripta · 2025Article
- Article
- Aberrant phase separation is a common killing strategy of positively charged peptides in biology and human disease.bioRxiv : the preprint server for biology · 2023Article
- Resolving hidden pixels beyond the resolution limit of projection imaging by square aperture.Scientific reports · 2023Article
- Soft X-ray tomography to map and quantify organelle interactions at the mesoscale.Structure (London, England : 1993) · 2022Article
- A protocol for full-rotation soft X-ray tomography of single cells.STAR protocols · 2022Article
- Correlative 3D cryo X-ray imaging reveals intracellular location and effect of designed antifibrotic protein-nanomaterial hybrids.Chemical science · 2021Article
- Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells.Cell reports methods · 2021Article
- Three-dimensional imaging of mitochondrial cristae complexity using cryo-soft X-ray tomography.Scientific reports · 2020Article
- Assaying three-dimensional cellular architecture using X-ray tomographic and correlated imaging approaches.The Journal of biological chemistry · 2020Review
- Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
The diversity of living cells, in both size and internal complexity, calls for imaging methods with adaptable spatial resolution. Soft x-ray tomography (SXT) is a three-dimensional imaging technique ideally suited to visualizing and quantifying the internal organization of single cells of varying sizes in a near-native state. The achievable resolution of the soft x-ray microscope is largely determined by the objective lens, but switching between objectives is extremely time-consuming and typically undertaken only during microscope maintenance procedures. Since the resolution of the optic is inversely proportional to the depth of focus, an optic capable of imaging the thickest cells is routinely selected. This unnecessarily limits the achievable resolution in smaller cells and eliminates the ability to obtain high-resolution images of regions of interest in larger cells. Here, we describe developments to overcome this shortfall and allow selection of microscope optics best suited to the specimen characteristics and data requirements. We demonstrate that switchable objective capability advances the flexibility of SXT to enable imaging cells ranging in size from bacteria to yeast and mammalian cells without physically modifying the microscope, and we demonstrate the use of this technology to image the same specimen with both optics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.