Evidence map›Paper›PMID 31977200›Full record

ArticleACS synthetic biology2020

Active Analyte Import Improves the Dynamic Range and Sensitivity of a Vitamin B

Monica P McNerney, Fernanda Piorino, Cirstyn L Michel, Mark P Styczynski

Open access · greenAbstract read
In one paragraph

Article in ACS synthetic biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 31 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. An Adenosylcobalamin Specific Whole-Cell Biosensor.Advanced healthcare materials · 2023
    Article
  9. Article
  10. Article
  11. HarnessingACS synthetic biology · 2022
    Article
  12. Low-cost, point-of-care biomarker quantification.Current opinion in biotechnology · 2022
    Review
  13. Systems biology-based analysis of cell-free systems.Current opinion in biotechnology · 2022
    Review
  14. Article
  15. Review
  16. Article
  17. Metabolic Dynamics inACS synthetic biology · 2021
    Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Monica P McNerneySchool of Chemical & Biomolecular Engineering , Georgia Institute of Technology , 311 Ferst Drive NW , Atlanta , Georgia 30332-0100 , United States.ORCID 0000-0001-7485-9211
Fernanda PiorinoSchool of Chemical & Biomolecular Engineering , Georgia Institute of Technology , 311 Ferst Drive NW , Atlanta , Georgia 30332-0100 , United States.
Cirstyn L MichelSchool of Chemical & Biomolecular Engineering , Georgia Institute of Technology , 311 Ferst Drive NW , Atlanta , Georgia 30332-0100 , United States.
Mark P StyczynskiSchool of Chemical & Biomolecular Engineering , Georgia Institute of Technology , 311 Ferst Drive NW , Atlanta , Georgia 30332-0100 , United States.ORCID 0000-0002-1479-6658
Georgia Institute of Technology · US

Funding

Modeling metabolic dynamics and regulation in biological systemsR35GM119701 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI STYCZYNSKI, MARK PHILIP-WALTER · 2016 to 2020
$1.6M
Synthetic biology-based detection of micronutrients with minimal equipmentR01EB022592 · NIBIB · GEORGIA INSTITUTE OF TECHNOLOGY · PI STYCZYNSKI, MARK PHILIP-WALTER · 2017 to 2020
$1.4M
NIBIB NIH HHS R01 EB022592NIGMS NIH HHS R35 GM119701
6 · The paper itself

Abstract

Cell-free systems provide a versatile platform for the development of low-cost, easy-to-use sensors for diverse analytes. However, sensor affinity dictates response sensitivity, and improving binding affinity can be challenging. Here, we describe efforts to address this problem while developing a biosensor for vitamin B

Indexed as

Biosensing TechniquesCell-Free SystemEscherichia coliEscherichia coli ProteinsGreen Fluorescent ProteinsHumansRiboswitchSpectrometry, FluorescenceTranscription FactorsVitamin B 12Escherichia coli ProteinsEutR protein, E coliGreen Fluorescent ProteinsRiboswitchTranscription FactorsVitamin B 12cell-free sensorscircuit tuningdiagnosticswhole-cell sensors

Identifiers

PMID31977200
PMCPMC7186122
OpenAlexW3002593086

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.