Evidence map›Paper›PMID 31975303›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Visualizing Protein Associations in Living Arabidopsis Embryo.

Yuchen Long, Yvonne Stahl, Stefanie Weidtkamp-Peters, Ikram Blilou

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yuchen LongLaboratoire Reproduction et Développement des Plantes (RDP), Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, Lyon, France.
Yvonne StahlInstitute for Developmental Genetics, Heinrich-Heine University, Universitätsstr. 1, Düsseldorf, Germany.
Stefanie Weidtkamp-PetersCenter for Advanced Imaging, Heinrich Heine University Düsseldorf, Universitätsstraße 1, Düsseldorf, Germany.
Ikram BlilouKing Abdullah University of Science and Technology (KAUST), Biological and Environmental Sciences and Engineering (BESE), Thuwal, Saudi Arabia. ikram.blilou@kaust.edu.sa.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein-protein interactions (PPI) are essential for a plethora of biological processes. These interactions can be visualized and quantified with spatial resolution using Förster resonance energy transfer (FRET) measured by fluorescence lifetime imaging microscopy (FLIM) technology. Currently, FRET-FLIM is routinely used in cell biology, and it has become a powerful tool to map protein interactions in native environments. However, implementing this technology in living multicellular organism remains challenging, especially when dealing with developing plant embryos where tissues are confined in multiple cell layers preventing direct imaging. In this chapter, we describe a step-by-step protocol for studying PPI using FRET-FLIM of the two transcription factors SCARECROW and SHORTROOT in Arabidopsis embryos. We provide a detailed description from embryo isolation to data analysis and representation.

Indexed as

ArabidopsisArabidopsis ProteinsFluorescence Resonance Energy TransferMicroscopy, FluorescenceProtein Interaction MappingProtein Interaction MapsSeedsTranscription FactorsArabidopsis ProteinsSCR protein, ArabidopsisTranscription FactorsEmbryoFluorescence lifetimeFRET-FLIMProtein interactionsSCR-SHR

Identifiers

PMID31975303

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.