ArticleMethods in molecular biology (Clifton, N.J.)2020
EMS Mutagenesis of Arabidopsis Seeds.
Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Novel genome editing approaches to manipulate apical meristem activity for crop yield.Frontiers in plant science · 2026Review
- Phenotypic and transcriptomic analysis reveals key genes associated with plant height in rubber tree and functional characterization of the candidate gene HbFLA11.The plant genome · 2025Article
- Directed mutagenesis in fruit crops.3 Biotech · 2025Review
- Genetic suppressor ofFrontiers in plant science · 2025Article
- Mapping of dwarfing gene and identification of mutant allele on plant height in wheat.Molecular breeding : new strategies in plant improvement · 2024Article
- Article
- Genomic variation induced by a low concentration of ethyl methanesulfonate (EMS) in quinoa 'Longli-4' variety.Botanical studies · 2024Article
- Article
- Genetic Screening of Factors in the Plant Protein Secretion.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Mutation in BrGGL7 gene encoding a GDSL esterase / lipase causes male sterility in Chinese cabbage (Brassica rapa L. ssp. pekinensis).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Screening of Induced Mutants Led to the Identification of Starch Biosynthetic Genes Associated with Improved Resistant Starch in Wheat.International journal of molecular sciences · 2022Article
- Current trends and insights on EMS mutagenesis application to studies on plant abiotic stress tolerance and development.Frontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ethylating agent ethyl methanesulfonate (EMS) is widely used for inducing random point mutations. In Arabidopsis, treatment with EMS causes GC-to-AT transitions with great efficiency: it has been estimated that a population of 50,000 well-mutagenized plants harbors one or more transitions in almost every GC pair of the genome. These properties, combined with ease of use, make EMS a mutagen of choice for genetic screens. Here, we describe a protocol for mutagenizing Arabidopsis seed with EMS. In addition, we briefly consider the germ line sectors typically induced by this treatment, and approaches for estimating the rate of induced mutations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.