ArticleMolecular medicine reports2020
Affinity improvement of the fully human anti‑TSLP recombinant antibody.
Article in Molecular medicine reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed, 4 citations in OpenAlex.
- A SARS-CoV-2 neutralizing antibody discovery by single cell sequencing and molecular modeling.Journal of biomedical research · 2022Article
- Novel Anti-Cytokine Strategies for Prevention and Treatment of Respiratory Allergic Diseases.Frontiers in immunology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thymic stromal lymphopoietin (TSLP) is a potentially important target for the treatment of asthma and malignancies. However, a fully human antibody reactive with TSLP is currently unavailable for clinical use. In a previous study, a human anti‑TSLP‑single‑chain antibody variable fragment (anti‑TSLP‑scFv) 84 was selected by phage display from a constructed human scFv library. In the present study, a computer simulation method was developed using Discovery Studio 4.5 software, to increase the affinity of anti‑TSLP‑scFv‑84. Specific primers were designed and mutated DNA sequences of anti‑TSLP‑scFvs were obtained by overlap extension PCR. The mutant scFvs were expressed in pLZ16 and affinity‑enhanced anti‑TSLP‑scFv‑M4 was screened using ELISA. However, in general the scFvs have low stability and short half‑lives in vivo. Therefore, scFv‑84 and scFv‑M4 were inserted into eukaryotic expression vectors (pcDNA3.1‑sp‑Fc and PMH3EN‑sp‑Fc) and then transfected into 293F cells to express anti‑TSLP‑scFv‑Fc. ELISA and western blotting results indicated the size of the anti‑TSLP‑scFv‑Fc to be ~50 kDa. Binding of anti‑TSLP‑scFv‑Fc‑M4 to TSLP was enhanced compared with the pre‑mutated scFv‑Fc‑84. The affinity of the mutated recombinant antibody was determined using the BIAcore technique and found to be ~10‑fold greater than the pre‑mutated antibody.
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Registered trials
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