Evidence map›Paper›PMID 31974596›Full record

ArticleMolecular medicine reports2020

Class C1 decoy oligodeoxynucleotide inhibits profibrotic genes expression in rat hepatic stellate cells.

Chun Rao, Yi-Ran Ni, Yan-Min Zhao, Yan-Qiong Zhang, Rui-Ting Zhou, Chang-Bai Liu, Lin Han, Jiang-Feng Wu

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Chun Rao *Department of Pathology, The People's Hospital of China Three Gorges University and the First People's Hospital of Yichang, Yichang, Hubei 443000, P.R. China.
Yi-Ran Ni *Department of Anatomy and Histology, Medical College, China Three Gorges University, Yichang, Hubei 443002, P.R. China.
Yan-Min ZhaoDepartment of Anatomy and Histology, Medical College, China Three Gorges University, Yichang, Hubei 443002, P.R. China.
Yan-Qiong ZhangDepartment of Anatomy and Histology, Medical College, China Three Gorges University, Yichang, Hubei 443002, P.R. China.
Rui-Ting ZhouDepartment of Anatomy and Histology, Medical College, China Three Gorges University, Yichang, Hubei 443002, P.R. China.
Chang-Bai LiuDepartment of Anatomy and Histology, Medical College, China Three Gorges University, Yichang, Hubei 443002, P.R. China.
Lin HanDepartment of Pathology, The People's Hospital of China Three Gorges University and the First People's Hospital of Yichang, Yichang, Hubei 443000, P.R. China.
Jiang-Feng WuDepartment of Pathology, The People's Hospital of China Three Gorges University and the First People's Hospital of Yichang, Yichang, Hubei 443000, P.R. China.
China Three Gorges University · CNYichang Central People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of the present study was to investigate whether class C1 decoy oligodeoxynucleotides (ODNs) can inhibit the expression of pro‑fibrotic genes associated with rat hepatic stellate cell (HSC) activation and hepatic fibrosis. Luciferase reporter assays were performed to test the promoter activities of transforming growth factor (TGF)‑β and its downstream target genes following transfection of decoy ODNs and plasmids into HSC‑T6 cells, and western blot assays were performed to measure the protein expression of those genes following decoy ODN transfection. Class C1 decoy ODNs were confirmed to inhibit the promoter activity of TGF‑β and its downstream target genes, such as type 1 collagen (COLI)α1, tissue inhibitor of metalloproteinases (TIMP)1 and α‑smooth muscle actin by Gaussia luciferase reporter assay, and to further downregulate the expression of TGF‑β, SMAD3, COLIα1 and TIMP1 by western blotting in activated HSC‑T6 cells. In conclusion, class C1 decoy ODNs inhibited pro‑fibrotic gene expression in rat HSCS by downregulating TGF‑β signaling.

Indexed as

Gene Expression RegulationAnimalsCell LineCollagen Type IDown-RegulationHepatic Stellate CellsLiver CirrhosisOligodeoxyribonucleotidesPromoter Regions, GeneticRatsSmad3 ProteinTissue Inhibitor of Metalloproteinase-1Transcription Factor HES-1Transforming Growth Factor betaCollagen Type IHes1 protein, ratOligodeoxyribonucleotidesSmad3 ProteinTissue Inhibitor of Metalloproteinase-1Transcription Factor HES-1Transforming Growth Factor betaclass c1 decoy oligodeoxynucleotideshepatic stellate cellstransforming growth factor-β signaling

Identifiers

PMID31974596
PMCPMC6947877
OpenAlexW2995734716

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.