ArticleJournal of experimental botany2020
Symplasmic isolation marks cell fate changes during somatic embryogenesis.
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- FANS uncovers WOX2-associated early regulators of auxin-induced somatic embryogenesis in Arabidopsis.BMC plant biology · 2026Article
- Microscopic probing into plasmodesmata (PD) and PD-derived intercellular gateways (PdIGs): Beyond morphology.Protoplasma · 2026Review
- Modulation of calcium, callose synthesis, membrane permeability and pectin methyl-esterase activity affect cell wall composition and embryo yield during Brassica napus microspore embryogenesis.Annals of botany · 2025Article
- Development and Applications of Somatic Embryogenesis in Grapevine (Plants (Basel, Switzerland) · 2024Review
- Multi-scale mechanisms driving root regeneration: From regeneration competence to tissue repatterning.The Plant journal : for cell and molecular biology · 2024Review
- Article
- Epigenetic modifications and miRNAs determine the transition of somatic cells into somatic embryos.Plant cell reports · 2023Review
- The Impact of Gold Nanoparticles on Somatic Embryogenesis Using the Example ofInternational journal of molecular sciences · 2023Article
- Apoplastic and Symplasmic Markers of Somatic Embryogenesis.Plants (Basel, Switzerland) · 2023Review
- Calcium Dynamics, WUSCHEL Expression and Callose Deposition during Somatic Embryogenesis inPlants (Basel, Switzerland) · 2023Article
- Identification ofForestry research · 2023Article
- Calcium dynamics and modulation in carrot somatic embryogenesis.Frontiers in plant science · 2023Article
- Leaf vein patterning is regulated by the aperture of plasmodesmata intercellular channels.PLoS biology · 2022Article
- Auxin biosynthesis maintains embryo identity and growth during BABY BOOM-induced somatic embryogenesis.Plant physiology · 2022Article
- Analysis of the Distribution of Symplasmic Tracers During Zygotic and Somatic Embryogenesis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses.Methods in molecular biology (Clifton, N.J.) · 2022Article
- ABA signalling promotes cell totipotency in the shoot apex of germinating embryos.Journal of experimental botany · 2021Article
- Transcriptomes and DNA methylomes in apomictic cells delineate nucellar embryogenesis initiation in citrus.DNA research : an international journal for rapid publication of reports on genes and genomes · 2021Article
- The Rab Geranylgeranyl Transferase Beta Subunit Is Essential for Embryo and Seed Development inInternational journal of molecular sciences · 2021Article
- Cell Wall Composition and Structure Define the Developmental Fate of Embryogenic Microspores inFrontiers in plant science · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell-to-cell signalling is a major mechanism controlling plant morphogenesis. Transport of signalling molecules through plasmodesmata is one way in which plants promote or restrict intercellular signalling over short distances. Plasmodesmata are membrane-lined pores between cells that regulate the intercellular flow of signalling molecules through changes in their size, creating symplasmic fields of connected cells. Here we examine the role of plasmodesmata and symplasmic communication in the establishment of plant cell totipotency, using somatic embryo induction from Arabidopsis explants as a model system. Cell-to-cell communication was evaluated using fluorescent tracers, supplemented with histological and ultrastructural analysis, and correlated with expression of a WOX2 embryo reporter. We showed that embryogenic cells are isolated symplasmically from non-embryogenic cells regardless of the explant type (immature zygotic embryos or seedlings) and inducer system (2,4-dichlorophenoxyacetic acid or the BABY BOOM (BBM) transcription factor), but that the symplasmic domains in different explants differ with respect to the maximum size of molecule capable of moving through the plasmodesmata. Callose deposition in plasmodesmata preceded WOX2 expression in future sites of somatic embryo development, but later was greatly reduced in WOX2-expressing domains. Callose deposition was also associated with a decrease DR5 auxin response in embryogenic tissue. Treatment of explants with the callose biosynthesis inhibitor 2-deoxy-D-glucose supressed somatic embryo formation in all three systems studied, and also blocked the observed decrease in DR5 expression. Together these data suggest that callose deposition at plasmodesmata is required for symplasmic isolation and establishment of cell totipotency in Arabidopsis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.