ArticleParasites & vectors2020
Development of a gp60-subtyping method for Cryptosporidium felis.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 47 citations in OpenAlex.
- Zoonotic Transmission Assessment of Cryptosporidium spp. in Close Human-Pet Environments in Yunnan Province, China.Veterinary medicine and science · 2026Article
- Novel gp60 subtypes and genetic diversity of Cryptosporidium felis in domestic and stray cats from Central Europe.Parasites & vectors · 2025Article
- Deciphering a cryptic minefield: A guide toCurrent research in parasitology & vector-borne diseases · 2025Review
- A review of recentCurrent research in parasitology & vector-borne diseases · 2025Review
- Molecular genetic characterization ofFrontiers in parasitology · 2025Article
- Genetic characterizations of Cryptosporidium spp. from children with or without diarrhea in Wenzhou, China: high probability of zoonotic transmission.BMC microbiology · 2024Article
- Evidence of the Zoonotic Transmission ofPathogens (Basel, Switzerland) · 2023Article
- Detection of Endoparasites in Non-Native Raccoons from Central Italy.Veterinary sciences · 2023Article
- Molecular identification of Cryptosporidium, Giardia, and Blastocystis from stray and household cats and cat owners in Tehran, Iran.Scientific reports · 2023Article
- Molecular characteristics and zoonotic potential of enteric protists in domestic dogs and cats in Egypt.Frontiers in veterinary science · 2023Article
- Molecular Detection and Distribution ofTransboundary and emerging diseases · 2023Article
- Review
- The detection and phylogenetic characterization ofFrontiers in cellular and infection microbiology · 2023Article
- Experimental infection of cats withJournal of feline medicine and surgery · 2022Article
- Evaluation of Next-Generation Sequencing Applied toPathogens (Basel, Switzerland) · 2022Article
- Molecular prevalence and subtyping of Cryptosporidium spp. in fecal samples collected from stray cats in İzmir, Turkey.BMC veterinary research · 2022Article
- Editorial for the Special Issue: Diagnosis, Epidemiology and Transmission Dynamics ofPathogens (Basel, Switzerland) · 2022Article
- An Update on ZoonoticAnimals : an open access journal from MDPI · 2021Review
- Development and Application of aMicroorganisms · 2021Article
- SubtypingPathogens (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
6 authors at 6 institutions in 3 countries.
Funding
Abstract
backgroundFeline cryptosporidiosis is an increasing problem, especially in catteries. In humans, close contact with cats could be a potential source of infection although the risk of contracting cryptosporidiosis caused by Cryptosporidium felis is considered to be relatively low. Sequencing of the 60-kDa glycoprotein gene is a commonly used tool for investigation of the genetic diversity and transmission dynamics of Cryptosporidium species. However, until now the sequence of gp60 from C. felis has not been available and genotyping has been limited to less discriminatory markers, such as 18S rRNA, COWP and HSP70.
methodsWe have identified the gp60 orthologue within the genome sequence of C. felis, and used the sequence to design a nested PCR for subtyping purposes. A total of 128 clinical isolates of both feline and human origin, were used to evaluate the marker.
resultsSequence analysis revealed large variations between the different samples. The C. felis gp60 lack the characteristic serine-tract found in many other cryptosporidian orthologues, instead it has an insertion of variable length (361-742 nt). Also, two cases of suspected zoonotic transmission of C. felis between cats and humans were successfully confirmed.
conclusionsWe have identified the gp60 gene in C. felis and show how this highly variable marker can be used in epidemiological investigations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.