ArticlePrion2020
Cross-validation of the RT-QuIC assay for the antemortem detection of chronic wasting disease in elk.
Article in Prion, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- RT-QuIC detection of chronic wasting disease prions in third eyelids from white-tailed deer.Scientific reports · 2025Article
- Advancing Prion Diagnostics: RT-QuIC Applications in Peripheral Tissues.Sub-cellular biochemistry · 2025Review
- Tracking Prions by RT-QuIC: An Update.Sub-cellular biochemistry · 2025Review
- Article
- PMCA screening of retropharyngeal lymph nodes in white-tailed deer and comparisons with ELISA and IHC.Scientific reports · 2023Article
- Novel polymorphisms in the prion protein gene (PRNP) and stability of the resultant prion protein in different horse breeds.Veterinary research · 2023Article
- Identification of a novel risk factor for chronic wasting disease (CWD) in elk: S100G single nucleotide polymorphism (SNP) of the prion protein gene (PRNP).Veterinary research · 2023Article
- Ticks harbor and excrete chronic wasting disease prions.Scientific reports · 2023Article
- The Zoonotic Potential of Chronic Wasting Disease-A Review.Foods (Basel, Switzerland) · 2023Review
- No evidence of uptake or propagation of reindeer CWD prions in environmentally exposed sheep.Acta veterinaria Scandinavica · 2022Article
- Review
- Detection of chronic wasting disease in feces and recto-anal mucosal associated lymphoid tissues with RT-QuIC in a naturally infected farmed white-tailed deer herd.Frontiers in veterinary science · 2022Article
- Detection by real-time quaking-induced conversion (RT-QuIC), ELISA, and IHC of chronic wasting disease prion in lymph nodes from Pennsylvania white-tailed deer with specificJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- Article
- Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear.Scientific reports · 2021Article
- Hofmeister Effect in RT-QuIC Seeding Activity of Chronic Wasting Disease Prions.Frontiers in bioengineering and biotechnology · 2021Article
- Management of chronic wasting disease in ranched elk: conclusions from a longitudinal three-year study.Prion · 2020Article
Corrections and comments
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Authors and funding
17 authors at 7 institutions in 2 countries.
Funding
Abstract
Chronic wasting disease is a progressively fatal, horizontally transmissible prion disease affecting several members of the cervid species. Conventional diagnosis relies on ELISA or IHC evaluation using tissues collected post-mortem; however, recent research has focused on newly developed amplification techniques using samples collected antemortem. The present study sought to cross-validate the real-time quaking-induced conversion assay (RT-QuIC) evaluation of rectal biopsies collected from an elk herd with endemic CWD, assessing both binary positive/negative test results as well as relative rates of amplification between laboratories. We found that results were correlative in both categories across all laboratories performing RT-QuIC, as well as to conventional IHC performed at a national reference laboratory. A significantly higher number of positive samples were identified using RT-QuIC, with results seemingly unhindered by low follicle counts. These findings support the continued development and implementation of amplification assays in the diagnosis of prion diseases of veterinary importance, targeting not just antemortem sampling strategies, but post-mortem testing approaches as well.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.