ReviewInternational journal of molecular sciences2020
Extremely Low Leakage Expression Systems Using Dual Transcriptional-Translational Control for Toxic Protein Production.
Review in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Omics-Based Expression Cassette for Heterologous Protein Production inACS synthetic biology · 2026Article
- Optimization Strategy of Expression Vectors and Regulatory Elements for Enhanced Protein Production inInternational journal of molecular sciences · 2025Review
- MiR-ON-CRISPR: a microRNA-activated CRISPR-dCas9 system for precise gene therapy in living cells and mouse models of sepsis.Nucleic acids research · 2025Article
- Review
- Heterologous Expression of Difficult to Produce Proteins in Bacterial Systems.International journal of molecular sciences · 2024Article
- Illuminating the oral microbiome and its host interactions: tools and approaches for molecular microbiology studies.FEMS microbiology reviews · 2023Review
- Development of a xylose-inducible promoter and riboswitch combination system for manipulating gene expression inApplied and environmental microbiology · 2023Article
- Article
- Systematic Comparison and Rational Design of Theophylline Riboswitches for Effective Gene Repression.Microbiology spectrum · 2023Article
- A dual-regulation inducible switch system for microRNA detection and cell type-specific gene activation.Theranostics · 2023Article
- Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant BacteriumACS synthetic biology · 2022Article
- Integrase-mediated differentiation circuits improve evolutionary stability of burdensome and toxic functions in E. coli.Nature communications · 2022Article
- Engineering Toehold-Mediated Switches for Native RNA Detection and Regulation in Bacteria.Journal of molecular biology · 2022Review
- Metabolic engineering of Escherichia coli for efficient degradation of 4-fluorophenol.AMB Express · 2022Article
- An Unnatural Amino Acid-Regulated Growth Controller Based on Informational Disturbance.Life (Basel, Switzerland) · 2021Article
- Random and combinatorial mutagenesis for improved total production of secretory target protein in Escherichia coli.Scientific reports · 2021Article
- An inducible transposon mutagenesis approach for the intracellular human pathogenWellcome open research · 2021Article
- Construction of an IS-FreeFrontiers in bioengineering and biotechnology · 2021Article
- Low temperature bacterial expression of the neutral amino acid transporters SLC1A5 (ASCT2), and SLC6A19 (B0AT1).Molecular biology reports · 2020Article
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Authors and funding
1 author.
Funding
Abstract
Expression systems for highly toxic protein genes must be conditional and suppress leakage expression to almost zero because even faint leakage expression may kill host cells, inhibit host growth, and cause loss of plasmids containing the toxic protein genes. The most widely used conditional expression systems are controlled only at the transcriptional level, and complete suppression of leakage expression is challenging. Recent progress on translational control has enabled construction of dual transcriptional-translational control systems in which leakage expression is strongly suppressed. This review summarizes the principles, features, and practical examples of dual transcriptional-translational control systems in bacteria, and provides future perspectives on these systems.
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