ArticlePlants (Basel, Switzerland)2020
NADH-GOGAT Overexpression Does Not Improve Maize (
Article in Plants (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Volatile organic compounds from Bacillus sp. PAR690 Promote plant growth by regulating photosynthesis and nitrogen metabolism.BMC plant biology · 2026Article
- Differential Effects of Melatonin on Nitrogen Metabolism and Growth inPlants (Basel, Switzerland) · 2026Article
- High-throughput screening for biostimulant discovery: opportunities to enhance nitrogen use efficiency.Frontiers in plant science · 2026Review
- Managing Arsenic Pollution from Soil-Plant Systems: Insights into the Role of Biochar.Plants (Basel, Switzerland) · 2025Review
- Identification of Wheat Glutamate Synthetase Gene Family and Expression Analysis under Nitrogen Stress.Genes · 2024Article
- The Role of Glutamine Synthetase (GS) and Glutamate Synthase (GOGAT) in the Improvement of Nitrogen Use Efficiency in Cereals.Biomolecules · 2023Review
- The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Plants (Basel, Switzerland) · 2023Article
- 2-Oxoglutarate contributes to the effect of foliar nitrogen on enhancing drought tolerance during flowering and grain yield of soybean.Scientific reports · 2023Article
- Unlocking the potentials of nitrate transporters at improving plant nitrogen use efficiency.Frontiers in plant science · 2023Review
- Multiomic Approaches Reveal Hormonal Modulation and Nitrogen Uptake and Assimilation in the Initial Growth of Maize Inoculated withPlants (Basel, Switzerland) · 2022Article
- The source, level, and balance of nitrogen during the somatic embryogenesis process drive cellular differentiation.Planta · 2022Review
- Melatonin improves nitrogen metabolism during grain filling under drought stress.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- A vacuolar hexose transport is required for xylem development in the inflorescence stem.Plant physiology · 2022Article
- Novel plant breeding techniques to advance nitrogen use efficiency in rice: A review.GM crops & food · 2021Review
- Review
- Special Issue Editorial: Plant Nitrogen Assimilation and Metabolism.Plants (Basel, Switzerland) · 2021Article
- Beneficial soil-borne bacteria and fungi: a promising way to improve plant nitrogen acquisition.Journal of experimental botany · 2020Review
- Concurrent activation of OsAMT1;2 and OsGOGAT1 in rice leads to enhanced nitrogen use efficiency under nitrogen limitation.The Plant journal : for cell and molecular biology · 2020Article
- Targeting Nitrogen Metabolism and Transport Processes to Improve Plant Nitrogen Use Efficiency.Frontiers in plant science · 2020Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Maize plants overexpressing NADH-GOGAT were produced in order to determine if boosting 2-Oxoglurate production used as a carbon skeleton for the biosynthesis of amino acids will improve plant biomass and kernel production. The NADH-GOGAT enzyme recycles glutamate and incorporates carbon skeletons into the ammonium assimilation pathway using the organic acid 2-Oxoglutarate as a substrate. Gene pyramiding was then conducted with NAD-IDH and NADH-GDH, two enzymes also involved in the synthesis of 2-Oxoglurate. NADH-GOGAT overexpression was detrimental for shoot biomass production but did not markedly affect kernel yield. Additional NAD-IDH and NADH-GDH activity did not improve plant performance. A decrease in kernel production was observed when NADH-GDH was pyramided to NADH-GOGAT and NAD-IDH. This decrease could not be restored even when additional cytosolic GS activity was present in the plants overexpressing the three enzymes producing 2-Oxoglutarate. Detailed leaf metabolic profiling of the different transgenic plants revealed that the NADH-GOGAT over-expressors were characterized by an accumulation of amino acids derived from glutamate and a decrease in the amount of carbohydrates further used to provide carbon skeletons for its synthesis. The study suggests that 2-Oxoglutarate synthesis is a key element acting at the interface of carbohydrate and amino acid metabolism and that its accumulation induces an imbalance of primary carbon and nitrogen metabolism that is detrimental for maize productivity.
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Registered trials
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