Evidence map›Paper›PMID 31972214›Full record

ArticleJournal of immunological methods2020

A method to study protein biomarkers in saliva using an automated capillary nano-immunoassay platform (Wes™).

Eduardo Reyes-Serratos, Marcelo Marcet-Palacios, David Rosenfield, Thomas Ritz, A Dean Befus

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of immunological methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Eduardo Reyes-SerratosDepartment of Medicine, Alberta Respiratory Centre, University of Alberta, Edmonton, Alberta, Canada. Electronic address: reyesser@ualberta.ca.
Marcelo Marcet-PalaciosDepartment of Medicine, Alberta Respiratory Centre, University of Alberta, Edmonton, Alberta, Canada; Northern Alberta Institute of Technology, Edmonton, Alberta, Canada.
David RosenfieldDepartment of Psychology, Southern Methodist University, Dallas, TX, United States.
Thomas RitzDepartment of Psychology, Southern Methodist University, Dallas, TX, United States.
A Dean BefusDepartment of Medicine, Alberta Respiratory Centre, University of Alberta, Edmonton, Alberta, Canada.

Funding

A wearable asthma trigger monitoring system with integrated physiological monitorU01EB021952 · NIBIB · UNIVERSITY OF MARYLAND BALTIMORE COUNTY · PI MEURET, ALICIA ESPERANZA, RAO, GOVIND · 2015 to 2015
$2.0M
Reward Sensitivity as a Mechanism of Positive Affect Treatment for AnhedoniaR61MH115138 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CRASKE, MICHELLE G, MEURET, ALICIA ESPERANZA · 2018 to 2019
$1.7M
NIBIB NIH HHS U01 EB021952NIMH NIH HHS R61 MH115138
6 · The paper itself

Abstract

Traditional immunoprobing techniques like Western blot continue to play a crucial role in the discovery and validation of biomarkers. This technique suffers from several limitations that affect reproducibility and feasibility for large-scale studies. Modern immunoprobing techniques have addressed several of these limitations. Here we contrast the use of Western blot and an automated capillary nano-immunoassay (CNIA), Wes™. We provide evidence highlighting the methodological advantages of Wes™ over Western blot in the validation of a novel biomarker, Calcium-binding protein and spermatid-associated 1 (hCABS1). While Wes™ offers a faster, more consistent approach with lower requirements for sample and antibody volumes, variations in expected molecular weights and computational algorithms used to analyze the data must receive careful consideration and assessment. Our data suggests that CNIA approaches are likely to positively impact biomarker discovery and validation.

Indexed as

AdolescentAdultAutomation, LaboratoryBiomarkersBlotting, WesternCalcium-Binding ProteinsFemaleHumansImmunoassayMaleMiddle AgedNanotechnologyReproducibility of ResultsSalivaYoung AdultBiomarkersCABS1 protein, humanCalcium-Binding ProteinsBiomarker(s)CABS1Capillary nano-immunoassayImmunoprobingSalivaStressWestern blotWes™

Identifiers

PMID31972214
PMCPMC11416074

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.