ArticleBioinformatics (Oxford, England)2020
CytoSeg 2.0: automated extraction of actin filaments.
Article in Bioinformatics (Oxford, England), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Automatic extraction of actin networks in plants.PLoS computational biology · 2023Article
- Tracing and tracking filamentous structures across scales: A systematic review.Computational and structural biotechnology journal · 2023Review
- FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking.PloS one · 2023Article
- SFAlab: image-based quantification of mechano-active ventral actin stress fibers in adherent cells.Frontiers in cell and developmental biology · 2023Article
- Label-free monitoring of spatiotemporal changes in the stem cell cytoskeletons in time-lapse phase-contrast microscopy.Biomedical optics express · 2022Article
- Quantitative mapping of keratin networks in 3D.eLife · 2022Article
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Authors and funding
4 authors.
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Abstract
motivationActin filaments (AFs) are dynamic structures that substantially change their organization over time. The dynamic behavior and the relatively low signal-to-noise ratio during live-cell imaging have rendered the quantification of the actin organization a difficult task.
resultsWe developed an automated image-based framework that extracts AFs from fluorescence microscopy images and represents them as networks, which are automatically analyzed to identify and compare biologically relevant features. Although the source code is freely available, we have now implemented the framework into a graphical user interface that can be installed as a Fiji plugin, thus enabling easy access by the research community. AVAILABILITY AND IMPLEMENTATION: CytoSeg 2.0 is open-source software under the GPL and is available on Github: https://github.com/jnowak90/CytoSeg2.0. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
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Registered trials
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