Evidence map›Paper›PMID 31970944›Full record

ArticleJournal of biomedical optics2020

Multiphoton microscopy: a personal historical review, with some future predictions.

Colin J R Sheppard

Abstract readHistorical Article
In one paragraph

Article in Journal of biomedical optics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. TWINKLE: An open-source two-photon microscope for teaching and research.bioRxiv : the preprint server for biology · 2025
    Article
  2. Article
  3. Review
  4. Review
  5. Article
  6. Visualization of Nanocarriers and Drugs in Cells and Tissue.Handbook of experimental pharmacology · 2024
    Article
  7. Article
  8. Review
  9. Review
  10. Review
  11. In focus in HCB.Histochemistry and cell biology · 2022
    Article
  12. Review
  13. Article
  14. Review
  15. Review
  16. Label-Free Multiphoton Microscopy: Much More Than Fancy Images.International journal of molecular sciences · 2021
    Review
  17. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Colin J R SheppardIstituto Italiano di Tecnologia, Department of Nanophysics, Genova, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are considered. Different contrast mechanisms, including use of nanoparticles, are discussed. Two new promising techniques that can be applied to multiphoton microscopy are described.

Indexed as

ForecastingHistory, 20th CenturyHistory, 21st CenturyHumansMicroscopy, FluorescenceMicroscopy, Fluorescence, Multiphotonfocal modulation microscopyimage scanning microscopymultiphoton microscopysecond-harmonic generationtwo-photon fluorescence

Identifiers

PMID31970944
PMCPMC6974959

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.