Evidence map›Paper›PMID 31970831›Full record

ArticleJournal of clinical laboratory analysis2020

Circular RNA VRK1 correlates with favourable prognosis, inhibits cell proliferation but promotes apoptosis in breast cancer.

Yang Li, Hai Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical laboratory analysis, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 4 pooled it
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 4 syntheses or guidelines pooled it, 30 citations in OpenAlex.

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  14. circRNAs and their relationship with breast cancer: a review.World journal of surgical oncology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Yang LiDepartment of Thyroid and Breast Surgery, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Hai LiDepartment of Thyroid and Breast Surgery, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.ORCID https://orcid.org/0000-0002-9161-3966
Central Hospital of Wuhan · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThis study aimed to evaluate the correlation between the circular RNA VRK serine/threonine kinase 1 (circ-VRK1) and the clinicopathological features and survival outcomes of breast cancer patients and determine its effects on breast cancer cell proliferation and apoptosis.

methodsA total of 350 breast cancer tissues and 163 breast cancer adjacent tissues, as controls, were acquired from Specimen House. Circ-VRK1 expression was measured using qPCR. The correlations between circ-VRK1 expression and demographic characteristics, tumour features and overall survival were analysed. In vitro, the effects of circ-VRK1 on breast cancer cell proliferation and apoptosis were measured by upregulating and downregulating circ-VRK1 expression via plasmid transfection.

resultsCirc-VRK1 was downregulated in breast cancer tissues compared with adjacent tissues and represented a good value in distinguishing breast cancer tissues from the adjacent tissues. Circ-VRK1 was associated with smaller tumour size, reduced T stage and lower TNM stage, and circ-VRK1 was also an independent predictor of better overall survival. According to the in vitro experiments, circ-VRK1 expression was lower in breast cancer cell lines (including BT474, MDA-MB-453 and MDA-MB-231) than in a normal breast epithelial cell line (MCF10A), and circ-VRK1 inhibited cell proliferation but promoted cell apoptosis in MDA-MB-231 cells.

conclusionCirc-VRK1 is downregulated in tumour tissues and associated with reduced tumour stage as well as better survival, and it inhibits cell proliferation but promotes cell apoptosis in breast cancer.

Indexed as

ApoptosisBreast NeoplasmsCell Line, TumorCell ProliferationFemaleGene Expression Regulation, NeoplasticHumansMiddle AgedMultivariate AnalysisPrognosisProportional Hazards ModelsRNA, CircularSurvival AnalysisRNA, Circularbreast cancercell activitiesCirc-VRK1clinicopathological featuressurvival

Identifiers

PMID31970831
PMCPMC6977307
OpenAlexW3001647220

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.