Evidence map›Paper›PMID 31968310›Full record

ArticleMethods and applications in fluorescence2020

Wide-field time-gated SPAD imager for phasor-based FLIM applications.

Arin Ulku, Andrei Ardelean, Michel Antolovic, Shimon Weiss, Edoardo Charbon, Claudio Bruschini, Xavier Michalet

Abstract read
In one paragraph

Article in Methods and applications in fluorescence, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed.

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  5. AlliGator: Open Source Fluorescence Lifetime Imaging Analysis in G.bioRxiv : the preprint server for biology · 2025
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  16. Single-shot time-folded fluorescence lifetime imaging.Proceedings of the National Academy of Sciences of the United States of America · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Arin UlkuAQUA Lab, Ecole Polytechnique Fédérale de Lausanne (EPFL), Neuchâtel, Switzerland.ORCID 0000-0002-6719-6189
Andrei ArdeleanAQUA Lab, Ecole Polytechnique Fédérale de Lausanne (EPFL), Neuchâtel, Switzerland.ORCID 0000-0002-1343-6116
Michel AntolovicAQUA Lab, Ecole Polytechnique Fédérale de Lausanne (EPFL), Neuchâtel, Switzerland.ORCID 0000-0001-6155-6895
Shimon WeissDepartment of Chemistry & Biochemistry, University of California at Los Angeles (UCLA), Los Angeles, California, United States of America.ORCID 0000-0002-0720-5426
Edoardo CharbonAQUA Lab, Ecole Polytechnique Fédérale de Lausanne (EPFL), Neuchâtel, Switzerland.ORCID 0000-0002-0620-3365
Claudio BruschiniAQUA Lab, Ecole Polytechnique Fédérale de Lausanne (EPFL), Neuchâtel, Switzerland.ORCID 0000-0002-6636-6596
Xavier MichaletDepartment of Chemistry & Biochemistry, University of California at Los Angeles (UCLA), Los Angeles, California, United States of America.ORCID 0000-0001-6602-7693

Funding

Single-photon counting APD arrays for high-through-put single-molecule assaysR01GM095904 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GHIONI, MASSIMO ANTONIO, MICHALET, XAVIER · 2012 to 2016
$2.0M
NIGMS NIH HHS R01 GM095904
6 · The paper itself

Abstract

We describe the performance of a new wide area time-gated single-photon avalanche diode (SPAD) array for phasor-FLIM, exploring the effect of gate length, gate number and signal intensity on the measured lifetime accuracy and precision. We conclude that the detector functions essentially as an ideal shot noise limited sensor and is capable of video rate FLIM measurement. The phasor approach used in this work appears ideally suited to handle the large amount of data generated by this type of very large sensor (512 × 512 pixels), even in the case of small number of gates and limited photon budget.

Identifiers

PMID31968310
PMCPMC8827132

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.