Evidence map›Paper›PMID 31967701›Full record

ArticleCell proliferation2020

Cross talk between RNA N6-methyladenosine methyltransferase-like 3 and miR-186 regulates hepatoblastoma progression through Wnt/β-catenin signalling pathway.

Xichun Cui, Zhifang Wang, Jianhao Li, Jianming Zhu, Zhigang Ren, Dandan Zhang, Wei Zhao, Yingzhong Fan, Da Zhang, Ranran Sun

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
85citing papers in PubMed, 1 pooled it
7.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

85 citing papers in PubMed, 1 synthesis or guideline pooled it, 118 citations in OpenAlex.

  1. Pooled it
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  15. Hepatoblastoma.Nature reviews. Disease primers · 2025
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25 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Xichun CuiPediatric Surgery Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Zhifang WangEndocrinology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Jianhao LiPrecision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Jianming ZhuPrecision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Zhigang RenPrecision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Dandan ZhangPathology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Wei ZhaoPediatric Surgery Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Yingzhong FanPediatric Surgery Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Da ZhangPediatric Surgery Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Ranran SunPrecision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.ORCID https://orcid.org/0000-0001-8757-6114
First Affiliated Hospital of Zhengzhou University · CN

Funding

China Postdoctoral Science Foundation 2017M610463 and 2018M632814Engineering LaboratoryNational Engineering Laboratory for Internet Medical System and Application open fund project NELIMSA2018P03National Key Research and Development Program of China 2018YFC2000500National Natural Science Foundation of China 2018ZX10301201-008National Natural Science Foundation of China 81600506National Natural Science Foundation of China 81702346National S&T Major Project of China 2018ZX10301201-008Research and Development 2017M610463Research and Development 2018M632814Research and Development 2018YFC2000500Research and Development NELIMSA2018P03
6 · The paper itself

Abstract

objectivesN6-methyladenosine (m6A) is a ubiquitous epigenetic RNA modification that plays a pivotal role in tumour development and metastasis. In this study, we aimed to investigate the expression profiling, clinical significance, biological function and the regulation of m6A-related genes in hepatoblastoma (HB). MATERIALS AND

methodsThe mRNA and protein expression levels of m6A-related genes were analysed using Gene Expression Omnibus (GEO) and tissue microarray (TMA) cohort. Kaplan-Meier analysis was performed to evaluate the prognostic value of m6A-related genes in HB. Knockdown of m6A-related genes was conducted to analyse its function on cell proliferation, migration and invasion. Furthermore, bioinformatics analysis and experimental verification were used to explore the potential molecular mechanism and signalling pathway.

resultsWe found that most m6A-related genes were significantly upregulated in HB tumour tissues. High levels of methyltransferase-like 3 (METTL3, P = .013), YTHDF2 (P = .037) and FTO (P = .032) indicated poor clinical outcomes, and the upregulation of METTL3 was an independent prognostic factor in HB patients. Functional assays showed that knockdown of METTL3 could dramatically suppress the proliferation, migration and invasion of HB cells. In addition, METTL3 was identified to be a direct target of microRNA-186 (miR-186). Consistently, miR-186 was low expressed in HB tumour tissues. Moreover, overexpression of miR-186 significantly inhibited cell aggressive phenotype both in vitro and in vivo, while the inhibitory effect could be reversed by METTL3 overexpression. Mechanism study indicated that miR-186/METTL3 axis contributed to the progression of HB via the Wnt/β-catenin signalling pathway.

conclusionsM6A-related genes were frequently dysregulated in HB. miR-186/METTL3/Wnt/β-catenin axis might serve as novel therapeutic targets and prognostic biomarkers in HB.

Indexed as

AdenosineAnimalsDisease ProgressionGene Expression Regulation, NeoplasticHepatoblastomaHep G2 CellsHumansLiver NeoplasmsMaleMethyltransferasesMice, Inbred C57BLMice, NudeMicroRNAsPrognosisWnt Signaling PathwayAdenosineMethyltransferasesMETTL3 protein, humanMettl3 protein, mouseMicroRNAsMIRN186 microRNA, humanMIRN186 microRNA, mouseN-methyladenosinehepatoblastomaMETTL3miR-186N6-methyladenosineWnt/β-catenin

Identifiers

PMID31967701
PMCPMC7106953
OpenAlexW3001542796

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.