Evidence map›Paper›PMID 31966886›Full record

ArticleInternational journal of clinical and experimental pathology2017

Effects of lncRNA uc.48+ siRNA on the release of CGRP in the spinal cords of rats with diabetic neuropathic pain.

Wei Xiong, Liping Huang, Yulin Shen, Shu Guan, Lingkun He, Zhoujie Tong, Mengxia Tan, Lijuan Liu, Yun Gao

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
0.4field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Wei XiongAffiliated Stomatological Hospital of Nanchang University Nanchang, Jiangxi, P. R. China.
Liping HuangDepartment of Physiology, Medical College of Nanchang University Nanchang, Jiangxi, P. R. China.
Yulin ShenDepartment of Physiology, Medical College of Nanchang University Nanchang, Jiangxi, P. R. China.
Shu GuanDepartment of Physiology, Medical College of Nanchang University Nanchang, Jiangxi, P. R. China.
Lingkun HeAffiliated Stomatological Hospital of Nanchang University Nanchang, Jiangxi, P. R. China.
Zhoujie TongQueen Mary College of Grade 2013, Nanchang University Nanchang, Jiangxi, P. R. China.
Mengxia TanDepartment of Physiology, Medical College of Nanchang University Nanchang, Jiangxi, P. R. China.
Lijuan LiuAffiliated Stomatological Hospital of Nanchang University Nanchang, Jiangxi, P. R. China.
Yun GaoDepartment of Physiology, Medical College of Nanchang University Nanchang, Jiangxi, P. R. China.
Nanchang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Long noncoding RNA (lncRNA) and factors influencing lncRNA expression are related to the nervous system diseases. The aims of the project are to study the effect of lncRNA uc.48+ siRNA on calcitonin gene related peptide (CGRP) release in the spinal cords (SCs) of diabetic neuropathic pain (DNP) rats to identify its possible mechanism and to provide new experimental evidence for the prevention and treatment of DNP. Male Sprague-Dawley rats were used to create a DNP rat model by feeding the rats a high-fat and fructose diet in addition to an intraperitoneal injection of streptozocin. Fasting blood glucose (FBG), mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were measured to select the DNP rats. The DNP rats were randomly divided into the following 4 groups: (1) a normal control group (Control), (2) a DNP rats treated with saline group (DNP), (3) a DNP rats treated with uc.48+ siRNA group (DNP + uc.48+ siRNA) and (4) a DNP rats treated with scrambled siRNA group (DNP + scramble siRNA). After intrathecal injection of uc.48+ small interfering RNA, the MWT and TWL of the DNP group significantly decreased compared to the Control group, but after the injection of uc.48+ small interfering RNA, the MWT and TWL of the DNP rats significantly increased (P<0.01, ANOVA test). The application of the methods of qPCR and WB produced results that revealed that the expressions of lncRNA uc.48+, CGRP, IL-1β and TNF-α in the SCs of the DNP group were much higher than those in the Control group (P<0.01, ANOVA test), but the expressions of these molecules in the DNP + uc.48+ siRNA group significantly decreased compared with the DNP group (P<0.01, ANOVA test). The phosphorylations of p38 and ERK1/2 in the DNP group were significantly enhanced compared with the Control group, whereas uc.48+ siRNA significantly reduced the increased phosphorylations of p38 and ERK1/2 pathway in the SCs of the DNP rats (P<0.01, ANOVA test). ELISA results revealed that uc.48+ siRNA significantly decreased the high levels of IL-1β and TNF-α in the sera of the DNP rats (P<0.01, ANOVA test). Therefore, lncRNA uc.48+ may play an important role in the transmission of DNP by promoting the release of CGRP in the SC. Small interfering lncRNA uc.48+ might alleviate the hyperalgesia and allodynia of DNP rats by suppressing the release of CGRP in the SCs of DNP rats, which might inhibit the phosphorylations of p38 and ERK1/2 and suppress the release of IL-1β and TNF-α in the SCs of DNP rats.

Indexed as

calcitonin gene related peptideDiabetic neuropathic painlncRNA uc.48+spinal cord

Identifiers

PMID31966886
PMCPMC6965966
OpenAlexW3012767804

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.