ArticleInternational journal of clinical and experimental pathology2017
miR-135b-5p regulates human mesenchymal stem cell osteogenic differentiation by facilitating the Hippo signaling pathway.
Article in International journal of clinical and experimental pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.
- MicroRNAs-mediated regulation of the differentiation of dental pulp-derived mesenchymal stem cells: a systematic review and bioinformatic analysis.Stem cell research & therapy · 2023Pooled it
- Cullin-associated and neddylation-dissociated protein 1 (CAND1) promotes cardiomyocyte proliferation and heart regeneration by enhancing the ubiquitinated degradation of Mps one binder kinase activator 1b (Mob1b).Cell death and differentiation · 2025Article
- miR-135b: A key role in cancer biology and therapeutic targets.Non-coding RNA research · 2025Review
- Osteointegration of functionalised high-performance oxide ceramics: imaging from micro-computed tomography.Journal of orthopaedic surgery and research · 2024Article
- Fetal Brain-Derived Exosomal miRNAs from Maternal Blood: Potential Diagnostic Biomarkers for Fetal Alcohol Spectrum Disorders (FASDs).International journal of molecular sciences · 2024Article
- The preliminary study of exosomes derived from thymosin beta 4-treated adipose-derived stem cells in fat grafting.Genes & genomics · 2023Article
- Transit Amplifying Cells (TACs): a still not fully understood cell population.Frontiers in bioengineering and biotechnology · 2023Review
- Functionalised High-Performance Oxide Ceramics with Bone Morphogenic Protein 2 (BMP-2) Induced Ossification: An In Vivo Study.Life (Basel, Switzerland) · 2022Article
- Histomorphometry of Ossification in Functionalised Ceramics with Tripeptide Arg-Gly-Asp (RGD): An In Vivo Study.Life (Basel, Switzerland) · 2022Article
- Exosomal miR-335 derived from mature dendritic cells enhanced mesenchymal stem cell-mediated bone regeneration of bone defects in athymic rats.Molecular medicine (Cambridge, Mass.) · 2021Article
- Identification of a 3-miRNA Signature Associated With the Prediction of Prognosis in Nasopharyngeal Carcinoma.Frontiers in oncology · 2021Article
- miRNAs Related to Different Processes of Fracture Healing: An Integrative Overview.Frontiers in surgery · 2021Review
- Review
- High-throughput sequencing analysis of the expression profile of microRNAs and target genes in mechanical force-induced osteoblastic/cementoblastic differentiation of human periodontal ligament cells.American journal of translational research · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA multifunctional titanium surface with osteogeneic, angiogeneic and antibacterial properties is needed to improve the osseointegration and long-term survival of dental implants. Particularly, the switch between the differentiation of mesenchymal stem cells (MSCs) into osteogenic and adipogenic lineages is regulated by numerous miRNAs. However, the association between miR-135b-5p and the Hippo signaling pathway during osteogenesis has not been elucidated. In the present study, we demonstrate that miR-135b-5p facilitates the in vitro osteogenesis of human mesenchymal stem cells (hMSCs).
methodshMSCs and Human Calvarial Osteoblasts (HCO) cells were cultured in vitro, followed by the transfection of the miR-135b-5p mimic or inhibitor using Lipofectamine 2000. The target of miR-135b-5p was determined by bioinformatics analysis and luciferase assay. Cell viability was tested using the MTT assay. The osteogenesis level was evaluated by alizarin red staining. miRNA and mRNA expression levels were detected by real-time PCR. The protein levels were assessed by western blotting.
resultsmiR-135b-5p was shown to be highly expressed in osteoblasts compared with that in hMSCs. The overexpression of miR-135b-5p promotes hMSC proliferation and osteogenesis, whereas its knockdown causes the inhibition of these processes. Furthermore, aberrant expression of miR-135b-5p promotes both osteogenic and proliferation factors. We next showed that the Hippo signaling pathway was activated by miR-135b-5p transfection. Next, we found that large tumor suppressor 1 (LATS1) and MOB kinase activator 1B (MOB1B), key negative regulators of the HIPPO signaling pathway, are direct targets of miR-135b-5p. In addition, the knockdown of LATS1 or MOB1B led to an increase in TEAD activity.
conclusionmiR-135b-5p regulates osteogenesis by controlling LATS1 and MOB1B expression and subsequently activating the HIPPO signaling pathway.
Indexed as
Identifiers
31966624PMC6965292W3007979984What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.